Ethanol extract of Herpetospermum caudigerum Wall ameliorates psoriasis-like skin inflammation and promotes degradation of keratinocyte-derived ICAM-1 and CXCL9

作     者：Ya Zhong Bo-wen Zhang Jin-tao Li Xin Zeng Jun-xia Pei Ya-mei Zhang Yi-xi Yang Fu-lun Li Yu Deng Qi Zhao Ya Zhong;Bo-wen Zhang;Jin-tao Li;Xin Zeng;Jun-xia Pei;Ya-mei Zhang;Yi-xi Yang;Fu-lun Li;Yu Deng;Qi Zhao

作者机构：Engineering Research Center of Sichuan-Xizang Traditional Medicinal PlantChengdu UniversityChengdu 610106Sichuan ProvinceChina Institute of Cancer Biology and Drug DiscoveryChengdu UniversityChengdu 610106Sichuan ProvinceChina School of Food and Biological EngineeringChengdu UniversityChengdu 610106Sichuan ProvinceChina School of PharmacyChengdu UniversityChengdu 610106Sichuan ProvinceChina Key Laboratory of Clinical GeneticsAffiliated Hospital of Chengdu UniversityChengdu 610106Sichuan ProvinceChina Department of DermatologyYueyang Hospital of Integrated Traditional Chinese and Western MedicineShanghai University of Traditional Chinese MedicineShanghai 200437China School of Basic Medical SciencesChengdu UniversityChengdu 610106Sichuan ProvinceChina 

出 版 物：《Journal of Integrative Medicine》 (结合医学学报（英文版）)

年 卷 期：2023年第21卷第6期

页      面：584-592页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：supported by grants from the National Natural Science Foundation of China(Grant No.82174202,No.32270407 and No.82074428) Natural Science Foundation of Sichuan Province(Grant No.2022NSFSC0726) Innovative Team Projects of Shanghai Municipal Commission of Health(Grant No.2022CX001) Innovation Foundation of the Affiliated Hospital of Chengdu University(Grant No.CDFYCX202209) Major Research and Development Plan of Xizang Autonomous Region(Grant No.XZ202201ZY0031G) 

主　　题：Psoriasis Herpetospermum caudigerum wall Cytokines Degradation 

摘      要：Objective:To explore whether the ethanol extract of Herpetospermum caudigerum Wall(EHC),a Xizang medicinal plant traditionally used for treating liver diseases,can improve imiquimod-induced psoriasis-like skin ***:Immunohistochemistry and immunofluorescence staining were used to determine the effects of topical EHC use in vivo on the skin pathology of imiquimod-induced psoriasis in *** protein levels of interferon-γ(IFN-γ),tumor necrosis factor-a(TNF-a),and interleukin-17A(IL-17A)in mouse skin samples were examined using immunohistochemical *** vitro,IFN-γ-induced HaCaT cells with or without EHC treatment were used to evaluate the expression of keratinocyte-derived intercellular cell adhesion molecule-1(ICAM-1)and chemokine CXC ligand 9(CXCL9)using Western blotting and reverse transcription-quantitative polymerase chain *** protein synthesis inhibitor cycloheximide and proteasome inhibitor MG132 were utilized to validate the EHC-mediated mechanism underlying degradation of ICAM-1 and ***:EHC improved inflammation in the imiquimod-induced psoriasis mouse model and reduced the levels of IFN-γ,TNF-a,and IL-17A in psoriatic *** with EHC also suppressed ICAM-1 and CXCL9 in epidermal *** mechanistic studies revealed that EHC suppressed keratinocyte-derived ICAM-1 and CXCL9 by promoting ubiquitin–proteasome-mediated protein degradation rather than transcriptional *** primary compounds including ehletianol C,dehydrodiconiferyl alcohol,herpetrione,herpetin,herpetotriol,herpetetrone and herpetetrol were identified from the EHC using ultra-performance liquid chromatography-quadrupole-time of flight-mass ***:Topical application of EHC ameliorates psoriasis-like skin symptoms and improves the inflammation at the lesion sites.