Ocimum sanctum extract preserves neuronal echotexture and controls seizure in lithium-pilocarpine induced status epilepticus rats

作     者：Soumya Sucharita Pattnaik Sudhir Chandra Sarangi Deeksha Sharma Surabhi Sinha Tapas C Nag Aruna Nambirajan Manjari Tripathi Soumya Sucharita Pattnaik;Sudhir Chandra Sarangi;Deeksha Sharma;Surabhi Sinha;Tapas C Nag;Aruna Nambirajan;Manjari Tripathi

作者机构：Department of PharmacologyAll India Institute of Medical SciencesNew DelhiIndia Department of AnatomyAll India Institute of Medical SciencesNew DelhiIndia Department of PathologyAll India Institute of Medical SciencesNew DelhiIndia Department of NeurologyAll India Institute of Medical SciencesNew DelhiIndia 

出 版 物：《Asian Pacific Journal of Tropical Biomedicine》 (亚太热带生物医学杂志（英文版）)

年 卷 期：2023年第13卷第11期

页      面：479-487,I0009页

核心收录：

学科分类：1006[医学-中西医结合] 1002[医学-临床医学] 10[医学] 100602[医学-中西医结合临床] 

主　　题：Status epilepticus Ocimum sanctum Seizure protection Neuronal injury Electron microscopy 

摘      要：Objective:To investigate the effect of Ocimum sanctum hydroalcoholic extract(OSHE)on seizure control and neuronal injury in rats with lithium-pilocarpine-induced status epilepticus(SE).Methods:SE was induced by administering lithium chloride followed by pilocarpine 24 h later.OSHE was administered either alone or in combination with valproate(VPA)3 days before SE induction until 14 days post-SE induction.Seizure parameters were recorded on day 1(0-3 h),day 1-3 and day 4-14 post-SE.On day 14 post-SE,neurobehavioural tests(elevated plus maze and passive avoidance)were done followed by total antioxidant capacity,neuron-specific enolase,immunohistochemistry,and electron microscopic assessment in the hippocampus and cortex tissue.Results:OSHE+VPA provided more significant seizure protection(75%)than VPA(62.5%),OSHE(62.5%),or SE control(12.5%)(overall P=0.003).The latency to stage-3/4 seizures was increased and the number of stage-3/4 seizures was reduced in all treatment groups compared to the SE control group(P=0.002 and0.001,respectively).The OSHE+VPA group also had better memory retention than other treatment groups(P0.001)in the passive avoidance test.Total antioxidant capacity level was significantly higher and neuron-specific enolase was lower in the OSHE and OSHE+VPA groups compared to the SE control group.Electron microscopic study showed significant myelin sheath damage(67.5%,P0.05)and axonal degeneration(51.8%,P0.001)in the hippocampus of the SE control group,which were alleviated by OSHE or OSHE+VPA treatment.In immunohistochemical analysis,the OSHE,OSHE+VPA,and VPA groups had a significantly higher number of viable neurons and less neuronal loss compared to the SE control in the hippocampus(P0.001).Conclusions:OSHE either alone or in combination with VPA shows better seizure control by preservation of neuronal echotexture and reducing oxidative stress in the hippocampus.