P-aminobenzoic acid promotes retinal regeneration through activation of Ascl1a in zebrafish

作     者：Meihui He Mingfang Xia Qian Yang Xingyi Chen Haibo Li Xiaobo Xia Meihui He;Mingfang Xia;Qian Yang;Xingyi Chen;Haibo Li;Xiaobo Xia

作者机构：Eye Center of Xiangya HospitalCentral South UniversityChangshaHunan ProvinceChina Hunan Key Laboratory of OphthalmologyChangshaHunan ProvinceChina National Clinical Research Center for Geriatric DisordersXiangya HospitalCentral South UniversityChangshaHunan ProvinceChina 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究(英文版))

年 卷 期：2024年第19卷第8期

页      面：1849-1856页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100212[医学-眼科学] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China,Nos.81974134(to XX)and 82000895(to HL) National Key Research and Development Program of China,Nos.2021YFA1101200&2021YFA1101202 National Natural Science Foundation of Hunan Province,China,No.2022JJ30071(to HL) 

主　　题：Achaetescute complex-like 1a(Ascl1a) metabolomics Müller glia p-aminobenzoic acid(PABA) retina regeneration zebrafish 

摘      要：The retina of zebrafish can regenerate completely after injury.M ultiple studies have demonstrated that metabolic alte rations occur during retinal damage;however to date no study has identified a link between metabolites and retinal regeneration of ***,we performed an unbiased metabolome sequencing in the N-methyl-D-aspartic acid-damaged retinas of zebrafish to demonstrate the metabolomic mechanism of retinal *** the differentially-ex pressed metabolites,we found a significant decrease in p-aminobenzoic acid in the N-methyl-D-aspartic acid-damaged retinas of ***,we investigated the role of p-aminobenzoic acid in retinal regeneration in adult *** rtantly,p-aminobenzoic acid activated Achaetescute complex-like 1a expression,thereby promoting Müller glia reprogramming and division,as well as Müller glia-derived progenitor cell ***,we eliminated folic acid and inflammation as downstream effectors of PABA and demonstrated that PABA had little effect on Müller glia *** together,these findings show that PABA contributes to retinal regeneration through activation of Achaetescute complex-like 1a expression in the N-methyl-Daspartic acid-damaged retinas of zebrafish.