Electroacupuncture at Sensitized Acupoints Relieves Somatic Referred Pain in Colitis Rats by Inhibiting Sympathetic-Sensory Coupling to Interferewith 5-HT Signaling Pathway
作者机构:School of Clinical MedicineChengdu Medical CollegeChengdu(610500)China Department of Rehabilitation Medicinethe First Affliated Hospital of Chengdu Medical CollegeChengdu(610500)China Department of Rehabilitation MedicineWest China HospitalSichuan University/West China School of NursingSichuan UniversityChengdu(610041)China Department of Neurologythe First Affiliated Hospital of Chengdu Medical CollegeChengdu(610500)China
出 版 物:《Chinese Journal of Integrative Medicine》 (中国结合医学杂志(英文版))
年 卷 期:2024年第30卷第2期
页 面:152-162页
核心收录:
学科分类:1005[医学-中医学] 1002[医学-临床医学] 100512[医学-针灸推拿学] 10[医学]
基 金:Supported by the National Natural Science Foundation of China(No.82004461) Medical Research Project of Sichuan Province(No.S21099) the First Affiliated Hospital of Chengdu Medical College(No.CYFY2021ZD05,No.CYFY-GQ27) Chengdu Medical College(No.CYZZD20-03,No.202013705082)
主 题:electroacupuncture sensitized acupoints sympathetic-sensory coupling 5-hydroxytryptamine neurogenic inflammation somaticreferredpain
摘 要:Objective:To investigate whether electroacupuncture(EA) at sensitized acupoints could reduce sympathetic-sensory coupling(SSC) and neurogenic inflammatory response by interfering with 5-hydroxytryptamine(5-HT)ergic neural pathways to relieve colitis and somatic referred pain,and explore the underlying ***:Rats were treated with 5% dextran sodium sulfate(DSS) solution for 7 days to establish a colitis *** rats were randomly divided into the control and model groups according to a random number table(n=6).According to the Research on Rat Acupoint Atlas,sensitized acupoints and non-sensitized acupoints were *** were randomly divided into the control,model,Zusanli-EA(ST 36),Dachangshu-EA(BL 25),and Xinshu(BL 15) groups(n=6),as well as the control,model,EA,and EA+GR113808(a 5-HT inhibitor)groups(n=6).The rats in the control group received no *** was administered on 2 days after modeling using the stimulation pavameters:1 mA,2 Hz,for 30 min,with sparse and dense waves,for 14 consecutive ***113808 was injected into the tail vein at 5 mg/kg before EA for 10 min for 7 consecutive *** sensitivity was assessed with von Frey *** weight and disease activity index(DAI) scores of rats were *** and eosin staining was performed to observe colon *** was analyzed by immunofluorescence *** staining was performed to detect 5-HT and substance P(SP) *** calcitonin gene-related peptide(CGRP) in skin tissue and tyrosine hydroxylase(TH) protein levels in DRG were detected by Western *** levels of hyaluronic acid(HA),bradykinin(BK),prostaglandin I2(PGI2) in skin tissue,5-HT,tryptophan hydroxylase 1(TPH1),serotonin transporters(SERT),5-HT 3 receptor(5-HT3R),and 5-HT 4 receptor(5-HT4R) in colon tissue were measured by enzyme-linked immunosorbent assay(ELISA).Results:BL 25 and ST 36 acupoints were determined as sensitized acupoints,and BL 1