Nasopharyngeal Local Transcriptional Profile upon SARS-CoV-2 Omicron BA.2.2 Breakthrough Infection

作     者：Yi Zhang Yang Zhou Yumeng Zhang Jiazhen Chen Jing Wu Peng Cui Shiyong Wang Yuanyuan Xu Zhongliang Shen Tao Xu Yang Li Jingwen Ai Ning Jiang Chao Qiu Wenhong Zhang Yi Zhang;Yang Zhou;Yumeng Zhang;Jiazhen Chen;Jing Wu;Peng Cui;Shiyong Wang;Yuanyuan Xu;Zhongliang Shen;Tao Xu;Yang Li;Jingwen Ai;Ning Jiang;Chao Qiu;Wenhong Zhang

作者机构：Department of Infectious DiseasesShanghai Key Laboratory of Infectious Diseases and Biosafety Emergency ResponseNational Medical Center for Infectious DiseasesHuashan HospitalShanghai Medical CollegeFudan UniversityShanghai 200040China School of Life SciencesFudan UniversityShanghai 200438China Institutes of Biomedical SciencesFudan UniversityShanghai 200032China National Clinical Research Center for Aging and MedicineHuashan HospitalFudan UniversityShanghai 200040China Shanghai Huashen Institute of Microbes and InfectionsShanghai 200052China 

出 版 物：《Infectious Diseases & Immunity》 (感染性疾病与免疫（英文）)

年 卷 期：2023年第3卷第4期

页      面：186-191页

学科分类：1002[医学-临床医学] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China(Nos 92169212 and 82161138018) Shanghai Key Laboratory of Infectious Diseases and Biosafety Emergency Response(20dz2260100) Key Discipline Construction Plan fromShanghaiMunicipalHealth Commission(GWV-10.1-XK01) ShanghaiMunicipal Science and Technology Major Project(HS2021SHZX001). 

主　　题：SARS-CoV-2 Local immune response Transcriptome Inflammation 

摘      要：The Omicron variants have continued to cause severe acute respiratory syndrome coronavirus 2 infections.To better understand the anti-viral effects of vaccination on host-virus interactions during the outbreak of BA.2.2 Omicron,we conducted RNA-seq transcriptome analysis on nasopharyngeal swabs from COVID-19 patients in Shanghai.This study was performed on selected cases from unvaccinated,fully vaccinated,and booster groups with the same founder virus infection background.We observed predominant immune cell chemotaxis and interleukin-1 production,as well as mucosal keratinization and epidermis development,in unvaccinated patients.In contrast,fully vaccinated subjects exhibited an obvious T-cell activation in the local immune response,whereas B-cell activation was higher in booster-vaccinated cases.In conclusion,our findings suggest that full or booster vaccination provides better adaptive immunity and relieve inflammation at the nasopharyngeal site,thereby reducing the risk of cytokine storm during breakthrough infection.