Investigating formate tolerance mechanisms in Saccharomyces cerevisiae and its application

作     者：Kai Wang Yining Liu Zhuoheng Wu Yilu Wu Haoran Bi Yanhui Liu Meng Wang Biqiang Chen Jens Nielsen Zihe Liu Tianwei Tan 

作者机构：National Energy R&D Center for BiorefneryBeijing Key Lab of BioprocessCollege of Life Science and TechnologyBeijing Advanced Innovation Center for Soft MatterScience and EngineeringBeijing University of Chemical TechnologyNo.15 North 3rd Ring Rd East100029 BeijingPR China Department of Biology and Biological EngineeringChalmers University of TechnologySE41296 GothenburgSweden 

出 版 物：《Green Carbon》 (绿碳（英文）)

年 卷 期：2023年第1卷第1期

页      面：65-74页

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070303[理学-有机化学] 0703[理学-化学] 

基　　金：the National Key R&D Program of China[2021YFC2103500] Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project[TSBICIP-KJGG-009] National Natural Science Foundation of China Beijing Advanced Innovation Center for Soft Matter Science and Engineering,Beijing University of Chemical Technology 

主　　题：Formate tolerance Adaptive laboratory evolution Saccharomyces cerevisiae Free fatty acids 

摘      要：Current global energy and environmental crisis have spurred efforts towards developing sustainable biotechnological solutions,such as utilizing CO_(2) and its derivatives as raw *** is an attractive onecarbon source due to its high solubility and low reduction ***,the regulatory mechanism of formate metabolism in yeast remains largely *** study employed adaptive laboratory evolution(ALE)to improve formate tolerance in Saccharomyces cerevisiae and characterized the underlying molecular *** evolved strain was applied to produce free fatty acids(FFAs)under high concentration of formate with glucose *** results showed that the evolved strain achieved a FFAs titer of 250 mg/***,this study sheds light on the regulatory mechanism of formate tolerance and provides a platform for future studies under high concentrations of formate.