Polydopamine-Based Colorimetric Superwettable Biosensor for Highly Sensitive Detection of Hydrogen Peroxide and Glucose

作     者：Jinze Li Yuhao Li Zhongfeng Gao 

作者机构：Key Laboratory of Interfacial Reaction and Sensing Analysis in Universities of ShandongSchool of Chemistry and Chemical EngineeringUniversity of JinanJinan 250022China College of Chemistry and Chemical EngineeringLinyi UniversityLinyi 276005China 

出 版 物：《Journal of Analysis and Testing》 (分析检测（英文）)

年 卷 期：2023年第7卷第2期

页      面：118-127页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 07[理学] 08[工学] 080202[工学-机械电子工程] 070302[理学-分析化学] 0802[工学-机械工程] 0703[理学-化学] 

基　　金：the financial support of the National Natural Science Foundation of China(22176080) SRT Program of University of Jinan(Yuhao Li) 

主　　题：Colorimetric assay Polydopamine Hydrogen peroxide Glucose detection Superwettable sensor 

摘      要：Superwettable surface has broad application prospects in fabricating biosensors due to its significant enrichment effect.Here,we report a polydopamine-based colorimetric superwettable sensor that integrates superhydrophobic-superhydrophilic micropatterns for the determination of hydrogen peroxide(H_(2)O_(2))and glucose.Dopamine can be oxidized into polydopamine with the addition of horseradish peroxidase(HRP)and H_(2)O_(2),leading to the deposited spots color change from colorless to black.The concentration of target can be determined by analyzing RGB value using a smartphone software.The superhydrophobic area on the superwettable surface helps capture droplets by confining them to superhydrophilic microwells.After droplet evaporation,the analytes are concentrated in the small superhydrophilic domain,thus greatly enhancing the sensitivity.The experimental results manifested that superwettable sensor is able to detect H_(2)O_(2)with a broad linear range of 0.25μmol/L-25 mmol/L and a low limit of detection(LOD)of 0.25μmol/L by naked eye.For glucose detection,the linear range of the sensor is from 2μmol/L to 20 mmol/L and LOD is 0.69μmol/L.The superwettable sensor has been successfully applied in practical samples,including cancerous cells,milk,urine,and human serum samples with acceptable results.This superwettable sensor has several merits,such as high sensitivity,rapid response,and low sample volume in a single microdroplet,and shows great potential in manufacturing portable devices for complex biosensing applications.