In Situ Deposition of Drug and Gene Nanoparticles on a Patterned Supramolecular Hydrogel to Construct a Directionally Osteochondral Plug

作     者：Jiawei Kang Yaping Li Yating Qin Zhongming Huang Yifan Wu Long Sun Cong Wang Wei Wang Gang Feng Yiying Qi Jiawei Kang;Yaping Li;Yating Qin;Zhongming Huang;Yifan Wu;Long Sun;Cong Wang;Wei Wang;Gang Feng;Yiying Qi

作者机构：Department of Orthopaedic SurgeryThe Second Affiliated HospitalZhejiang University School of MedicineHangzhou City 310009Zhejiang ProvincePeople’s Republic of China College of Chemical and Biological EngineeringZhejiang UniversityHangzhou 310027People’s Republic of China ZJU-Hangzhou Global Scientific and Technological Innovation CenterHangzhou 311215ZhejiangPeople’s Republic of China The Affiliated Nanhua HospitalOrthopedic Research CentreHengyang Medical SchoolUniversity of South ChinaHengyang 421001HunanPeople’s Republic of China Department of RadiologyJining No.1 People’s HospitalJining Medical UniversityJining 272000ShandongPeople’s Republic of China 

出 版 物：《Nano-Micro Letters》 (纳微快报（英文版）)

年 卷 期：2024年第16卷第1期

页      面：341-359页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 0805[工学-材料科学与工程（可授工学、理学学位）] 070303[理学-有机化学] 0703[理学-化学] 0702[理学-物理学] 

基　　金：the Natural Science Foundation of China(Grant Nos.82072413,82101649) National Key Research and Development Program of China(Grant Nos.2021YFE0105400) 

主　　题：Osteochondral regeneration Oriented hydrogel Kartogenin miRNA-26a 

摘      要：The integrated repair of bone and cartilage boasts advantages for osteochondral restoration such as a long-term repair effect and less deterioration compared to repairing cartilage *** multifactorial,spatially oriented scaffolds to stimulate osteochondral regeneration,has immense ***,targeted drugs,namely kartogenin@polydopamine(KGN@PDA)nanoparticles for cartilage repair and miRNA@calcium phosphate(miRNA@CaP)NPs for bone regeneration,were in situ deposited on a patterned supramolecular-assembled 2-ureido-4[lH]-pyrimidinone(UPy)modified gelation hydrogel film,facilitated by the dynamic and responsive coordination and complexation of metal ions and their *** hydrogel film can be rolled into a cylindrical plug,mimicking the Haversian canal structure of natural *** resultant hydrogel demonstrates stable mechanical properties,a self-healing ability,a high capability for reactive oxygen species capture,and controlled release of KGN and *** vitro,KGN@PDA and miRNA@CaP promote chondrogenic and osteogenic differentiation of mesenchymal stem cells via the JNK/RUNX1 and GSK-3β/β-catenin pathways,*** vivo,the osteochondral plug exhibits optimal subchondral bone and cartilage regeneration,evidenced by a significant increase in glycosaminoglycan and collagen accumulation in specific zones,along with the successful integration of neocartilage with subchondral *** biomaterial delivery approach represents a significant toward improved osteochondral repair.