Antioxidant and antiarthritic potential of berberine: In vitro and in vivo studies

作     者：Shweta Jain Shalini Tripathi Pushpendra Kumar Tripathi Shweta Jain;Shalini Tripathi;Pushpendra Kumar Tripathi

作者机构：Sir Madanlal Institute of PharmacyEtawah 206130India Rameshwaram Institute of Technology and ManagementDr.A.P.J.Abdul Kalam Technical UniversityLucknow 226001India Institute of Pharmaceutical SciencesUniversity of LucknowLucknow 226001India 

出 版 物：《Chinese Herbal Medicines》 (中草药（英文版）)

年 卷 期：2023年第15卷第4期

页      面：549-555页

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：North Carolina Central University  NCCU 

主　　题：antioxidant antiarthritic activity berberine Berberis aristata DC microwave assistance 

摘      要：Objective: To extract and isolate berberine from Berberis aristata(Berberidaceae). Isolated berberine was characterised using spectroscopy and its antioxidant and antiarthritic activity was ***: The berberine was isolated from B. aristata using microwave-assisted extraction(MAE) and characterised by a spectroscopic technique. The isolated berberine was evaluated for its antioxidant activity in DPPH, nitric oxide, and superoxide scavenging assays, while antiarthritic activity was evaluated in the complete freund’s adjuvant(CFA)-induced arthritis rat ***: The antioxidant activity of berberine revealed potent antioxidant activity in DPPH, nitric oxide,and superoxide scavenging assays. The in vivo antiarthritic activity of berberine in the CFA-induced arthritis rat model showed a significant reduction in paw diameter, arthritic score, and an increase in body weight. Furthermore, a concentration-dependent ameliorating action of berberine on haematological parameters was noticed. Proinfammatory biomarkers, including IL-6, IL-10, and TGF-b in serum were reported, and histopathology examination revealed that berberine decreased pannus formation, synovial hyperplasia, and bone erosion. Radiographic investigation showed soft tissue infammation, bone resorption and erosion, joint gap reduction, and substantial connective tissue expansion after treatment with ***: The ameliorating action on haematological parameters and proinfammatory biomarkers of berberine makes them a suitable remedy for the treatment of arthritis.