Simultaneous detection and differentiates of Brucella abortus and Brucella melitensis by combinatorial PCR

作     者：Reza Mirnejad Reza Hosseini Doust Reza Kachuei Seied Mojtaba Mortazavi Mehdi Khoobdel Ali Ahamadi 

作者机构：Molecular Biology Research CenterBaqiyatallah University of Medical SciencesTehranIran Health StationKerman University of Medical SciencesKermanIran Health Research CenterBaqiyatallah University of Medical SciencesTehranIran 

出 版 物：《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志（英文版）)

年 卷 期：2012年第5卷第1期

页      面：24-28页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 1001[医学-基础医学(可授医学、理学学位)] 100103[医学-病原生物学] 10[医学] 

基　　金：supported by Molecular Biology Research Center Baqiyatallah University of Medical Sciences with grant number BMSU/MBRC- 89-009 

主　　题：Brucella abortus Brucella melitensis Combinatorial PCR 

摘      要：Objective:To evaluate simultaneous detection and differentiates of Brucella abortus(***) and Brucella melitensis(***) through the combinatorial POR ***:This study was designed using three primers that could simultaneously identify and differentiate two major species of pathogenic Brucella in humans and *** and differentiation of each species using the size of the PCR product were *** determine the specifieity of the method,bacteria close to the genus Brucella were ***,to confirm PCR *** addition to the products sequence,RFLP was performed on PCR products using restriction ***:The method of optimized combinatorial PCR in this study could simultaneously detect and differentiate *** and *** with high specificity and sensitivity in clinical *** of species is based on the resulting bands: therefore,the band 494 bp for *** and 733 bp for *** were *** and sequencing results confirmed PCR ***:The results of this study shows that without routine diagnostic methods such as culture and serology tests,using the molecular method of combinatorial PCR,important species of Brucella can be simultaneously identified and differentiated in clinical samples.