Effect of the same mechanical loading on osteogenesis and osteoclastogenesis in vitro

作     者：Yong Guo Yang Wang Yinqin Liu Haitao Wang Chun Guo Xizheng Zhang Chaoyong Bei Guo Yong;Wang Yang;Liu Yinqin;Wang Haitao;Guo Chun;Zhang Xizheng;Bei Chaoyong

作者机构：College ofBioteehnology Guilin Medical University Guilin 541004 China Department of Medicine Luohe Medical College Luohe 462000 China Institute of Medical Equipment Academy of Military Medical Sciences Tianjin 300161 China Department of Orthopedic Affiliated Hospital of Guilin Medical University. Guilin 541000 China 

出 版 物：《Chinese Journal of Traumatology》 (中华创伤杂志（英文版）)

年 卷 期：2015年第18卷第3期

页      面：150-156页

核心收录：

学科分类：0710[理学-生物学] 0403[教育学-体育学] 1002[医学-临床医学] 07[理学] 08[工学] 0805[工学-材料科学与工程（可授工学、理学学位）] 071003[理学-生理学] 

基　　金：This work was financially supported by the National Natural Science Foundation of China （No.11372351  No.31370942  No.81160223）  and Scientific Research Foundation of Guangxi Higher Education （No.KY2015LX241）. 

主　　题：成骨细胞分化 破骨细胞 体外培养 机械负荷 RAW264.7细胞 骨髓间充质干细胞 基质金属蛋白酶-9 骨髓基质干细胞 

摘      要：Purpose: To investigate the influence of the same mechanical loading on osteogenesis and osteoclastogenesis in vitro . Methods: Primary osteoblasts, bone marrow-derived mesenchymal stem cells (BMSCs, cultured in osteoinductive medium) and RAW264.7 cells cultured in osteoclast inductive medium were all subjected to a 1000 μstrain (μs) at 1 Hz cyclic mechanical stretch for 30 min (twice a day). Results: After mechanical stimulation, the alkaline phosphatase (ALP) activity, osteocalcin protein level of the osteoblasts and BMSCs were all enhanced, and the mRNA levels of ALP and collagen type I increased. Additionally, extracellular-deposited calcium of both osteoblasts and BMSCs increased. At the same time, the activity of secreted tartrate-resistant acid phosphatase, the number of tartrate-resistant acid phosphatase-positive multinucleated cells, matrix metalloproteinase-9 protein levels of RAW264.7 cells and the extracellular calcium solvency all decreased. Conclusion: The results demonstrated that 1000 μs cyclic mechanical loading enhanced osteoblasts activity, promoted osteoblastic differentiation of BMSCs and restrained osteoclastogenesis of RAW264.7 cells in vitro .