Evolution of chemistry and selection technology for DNA-encoded library

作     者：Peixiang Ma Shuning Zhang Qianping Huang Yuang Gu Zhi Zhou Wei Hou Wei Yi Hongtao Xu 

作者机构：Shanghai Key Laboratory of Orthopedic ImplantsDepartment of OrthopedicsShanghai Ninth People’s HospitalShanghai Jiao Tong UniversitySchool of MedicineShanghai 200025China Shanghai Institute for Advanced Immunochemical StudiesShanghaiTech UniversityShanghai 201210China Guangzhou Municipal and Guangdong Provincial Key Laboratory of Molecular Target&Clinical PharmacologyThe NMPA and State Key Laboratory of Respiratory DiseaseGuangzhou Medical UniversityGuangzhou 511436China College of Pharmaceutical Science and Institute of Drug Development&Chemical BiologyZhejiang University of TechnologyHangzhou 310014China 

出 版 物：《Acta Pharmaceutica Sinica B》 (药学学报（英文版）)

年 卷 期：2024年第14卷第2期

页      面：492-516页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 10[医学] 

基　　金：Financial support was provided by the National Natural Science Foundation of China(grant numbers 22177073,21977070,21907085,and U19A2011) the Natural Science Foundation of Shanghai,China(grant numbers 21ZR1442900 and 23ZR1437600) the Natural Science Foundation of Zhejiang Province,China(grant number LY22H300001) Shanghai Frontiers Science Center of Degeneration and Regeneration in Skeletal System Shanghai Key Laboratory of Orthopedic Implants(grant number KFKT202207,China)for financial support 

主　　题：DNA-encoded library Drug discovery High-throughput selection DNA-compatible synthesis In vitro evolution Chemical central dogma 

摘      要：DNA-encoded chemical library(DEL)links the power of amplifiable genetics and the nonself-replicating chemical phenotypes,generating a diverse chemical *** analogy with the biological world,the DEL world can evolve by using a chemical central dogma,wherein DNA replicates using the PCR reactions to amplify the genetic codes,DNA sequencing transcripts the genetic information,and DNA-compatible synthesis translates into chemical ***,DNA-compatible synthesis is the key to expanding the DEL chemical ***,the evolution-driven selection system pushes the chemicals to evolve under the selective pressure,i.e.,desired selection *** this perspective,we summarized recent advances in expanding DEL synthetic toolbox and panning strategies,which will shed light on the drug discovery harnessing in vitro evolution of chemicals via DEL.