Circulating nucleic acids as liquid biopsies for disease prediction,screening and diagnosis

作     者：Yuqing Sun Yongling Tang Long Yu Yuhao Wang Shuang Peng Xiang Zhou 

作者机构：College of Chemistry and Molecular SciencesKey Laboratory of Biomedical Polymers-Ministry of EducationDepartment of Hematology of Zhongnan HospitalTaikang Center for Life and Medical SciencesWuhan UniversityWuhan 430072China 

出 版 物：《Science China Chemistry》 (中国科学(化学英文版))

年 卷 期：2023年第66卷第11期

页      面：3006-3023页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China(92153303,21721005,91940000 to X.Z) the National Science Foundation for Young Scientists of China(21907078 to S.P.)。 

主　　题：cliec acids liquid biopsy biomarkers 

摘      要：Liquid biopsy used molecular information in body liquid to perform early diagnosis,screening,monitoring,prognosis,and treatment of various diseases.Circulating free nucleic acids(cfNA)are important diagnostic biomarkers,providing a window to accurately and immediately observe the body s vital activity status.With the development of gene sequencing technology and bioinformatics technology,genetic,epigenetic,and fragtomics alterations that can be detected in cfDNA,as well as the expression level of miRNA and cf-mRNA can be quantified,this can reflect its tissue origin,gene regulation,genome evolution,and disease pathogenesis.This review focuses on the clinical utility of cfNA in different body liquids(blood,urine,bile),and discusses the diagnostic efficacy and accuracy of cfNA as diagnostic biomarkers in a variety of diseases.Blood is widely used to diagnose various tissue lesions for liquid biopsies as a body fluid circulating throughout the body,reflecting the state of the entire body.Bile and urine,as local circulating body fluids,can better reflect the changing state of tissues around the biliary tract and tissues around the bladder,respectively.In addition,normalized sample preservation,cfNA extraction,and detection procedures will help the practical application of cfNA in the clinic.