FgGyp8 as a putative FgRab1 GAP is required for growth and pathogenesis by regulating FgSnc1-mediated secretory vesicles fusion in Fusarium graminearum

作     者：ZHANG Xing-zhi CHEN Shuang Yakubu Saddeeq ABUBAKAR MAO Xu-zhao MIAO Peng-fei WANG Zong-hua ZHOU Jie ZHENG Hua-wei ZHANG Xing-zhi;CHEN Shuang;Yakubu Saddeeq ABUBAKAR;MAO Xu-zhao;MIAO Peng-fei;WANG Zong-hua;ZHOU Jie;ZHENG Hua-wei

作者机构：Fujian Key Laboratory on Conservation and Sustainable Utilization of Marine Biodiversity/Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait CropsMinjiang UniversityFuzhou 350108P.R.China Fujian University Key Laboratory for Plant-Microbe InteractionCollege of Life SciencesFujian Agriculture and Forestry UniversityFuzhou 350002P.R.China Department of BiochemistryFaculty of Life SciencesAhmadu Bello UniversityZaria 810211Nigeria 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2023年第22卷第11期

页      面：3444-3457页

核心收录：

学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 

基　　金：National Natural Science Foundation of China (31970141) the Natural Science Foundation of Fujian Province, China (2020J06047) the Foundation of Minjiang University, China (MJY19019) the Foundation of Fujian Agriculture and Forestry University, China (KFb22050XA) 

主　　题：Fusarium graminearum FgGyp8 GTPase-activating protein FgRab1 conidiogenesis pathogenicity 

摘      要：Fusarium graminearum is an important plant pathogenic fungus that causes disease and yield reduction in many cereal crops, such as wheat and barley. Gyp8 stimulates GTP hydrolysis on Ypt1 in yeast. However, the functions of Gyp8 in plant pathogenic fungi are still unknown. In this study, we investigated the roles of Fg Gyp8 in F. graminearum by genetic and pathological analyses. Through gene knockout and phenotypic analyses, we found that Fg Gyp8 is required for vegetative growth in F. graminearum. The conidiation, conidial size and number of septa per conidium of ΔFggyp8 mutant are significantly reduced when compared to the wild type PH-1. Furthermore, Fg Gyp8 is crucial for pathogenicity on wheat coleoptiles and wheat heads. Fg Gyp8 contains a conserved TBC domain. Domain deletion analysis showed that the TBC domain, C-and N-terminal regions of Fg Gyp8 are all important for its biological functions in F. graminearum. Moreover, we showed that Fg Gyp8 catalyzes the hydrolysis of the GTP on Fg Rab1 to GDP in vitro, indicating that Fg Gyp8 is a GTPase-activating protein(GAP) for Fg Rab1. In addition, we demonstrated that Fg Gyp8 is required for Fg Snc1-mediated fusion of secretory vesicles with the plasma membrane in F. graminearum. Finally, we showed that Fg Gyp8 has functional redundancy with another Fg Rab1 GAP, Fg Gyp1, in F. graminearum. Taken together, we conclude that Fg Gyp8 is required for vegetative growth, conidiogenesis, pathogenicity and acts as a GAP for Fg Rab1 in F. graminearum.