Intraoperative bioprinting of human adipose-derived stem cells and extra-cellular matrix induces hair follicle-like downgrowths and adipose tissue formation during full-thickness craniomaxillofacial skin reconstruction

作     者：Youngnam Kang Miji Yeo Irem Deniz Derman Dino JRavnic Yogendra Pratap Singh Mecit Altan Alioglu Yang Wu Jasson Makkar Ryan R.Driskell Ibrahim T.Ozbolat 

作者机构：Engineering Science and Mechanics DepartmentPenn State UniversityUniversity ParkPA16802USA The Huck Institutes of the Life SciencesPenn State UniversityUniversity ParkPA16802USA Department of SurgeryCollege of MedicinePenn State UniversityHersheyPA17033USA School of Mechanical Engineering and AutomationHarbin Institute of TechnologyShenzhen518055China School of Molecular BiosciencesCollege of Veterinary MedicineWashington State UniversityPullmanWA99164USA Department of Biomedical EngineeringPenn State UniversityUniversity ParkPA16802USA Materials Research InstitutePenn State UniversityUniversity ParkPA16802USA Department of NeurosurgeryPennsylvania State College of MedicineHersheyPA17033USA Penn State Cancer InstitutePenn State UniversityHersheyPA17033USA Department of Medical OncologyCukurova UniversityAdan101130Turkey 

出 版 物：《Bioactive Materials》 (生物活性材料（英文）)

年 卷 期：2024年第33卷第3期

页      面：114-128页

核心收录：

学科分类：1001[医学-基础医学(可授医学、理学学位)] 100101[医学-人体解剖与组织胚胎学] 10[医学] 

基　　金：supported by National Institutes of Health Award R01DE028614 R56HL157190 R21AR082668 and R01AR078743 and 2236 CoCirculation2 of TUBITAK award 121C359 

主　　题：Intraoperative bioprinting Adipose-derived extracellular matrix Adipose-derived stem cells Skin tissue engineering 

摘      要：Craniomaxillofacial(CMF)reconstruction is a challenging clinical *** often necessitates skin replacement in the form of autologous graft or flap surgery,which differ from one another based on hypodermal/dermal ***,both approaches are plagued by scarring,poor cosmesis,inadequate restoration of native anatomy and hair,alopecia,donor site morbidity,and potential for ***,new reconstructive approaches are warranted,and tissue engineered skin represents an exciting *** this study,we demonstrated the reconstruction of CMF full-thickness skin defects using intraoperative bioprinting(IOB),which enabled the repair of defects via direct bioprinting of multiple layers of skin on immunodeficient rats in a surgical *** a newly formulated patient-sourced allogenic bioink consisting of both human adipose-derived extracellular matrix(adECM)and stem cells(ADSCs),skin loss was reconstructed by precise deposition of the hypodermal and dermal components under three different sets of animal ***,even at a very low concentration such as 2%or less,has shown to be bioprintable via droplet-based bioprinting and exhibited de novo adipogenic capabilities both in vitro and in *** findings demonstrate that the combinatorial delivery of adECM and ADSCs facilitated the reconstruction of three full-thickness skin defects,accomplishing near-complete wound closure within two *** importantly,both hypodermal adipogenesis and downgrowth of hair follicle-like structures were achieved in this two-week time *** approach illustrates the translational potential of using human-derived materials and IOB technologies for full-thickness skin loss.