Chondrogenic medium in combination with a c-Jun N-terminal kinase inhibitor mediates engineered cartilage regeneration by regulating matrix metabolism and cell proliferation

作     者：Peiling Zhang Qianyi Wang Jie Chen Zheng Ci Wei Zhang Yu Liu Xiaoyun Wang Guangdong Zhou 

作者机构：Department of Plastic and Reconstructive SurgeryShanghai Key Laboratory of Tissue EngineeringShanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineShanghai200023China National Tissue Engineering Center of ChinaShanghai200241China Department of Research Institute of Plastic SurgeryWei Fang Medical CollegeWei FangShandong261041China Department of AnesthesiologyShanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineShanghai200023China Department of Plastic SurgeryTong Ren HospitalShanghai Jiao Tong University School of MedicineShanghai200050China 

出 版 物：《Regenerative Biomaterials》 (再生生物材料（英文版）)

年 卷 期：2023年第10卷第1期

页      面：1468-1480页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 08[工学] 0836[工学-生物工程] 

基　　金：supported by the National Key Research and Development Program of China(2017YFC1103900 and 2018YFC1105800) the National Natural Science Foundation of China(81871502 and 81701843) the Program of Shanghai Academic/Technology Research Leader(19XD1431100) the Shanghai Collaborative Innovation Program on Regenerative Medicine and Stem Cell Research(2019CXJQ01) the Clinical Research Plan of SHDC(No.SHDC2020CR2045B),Shanghai Municipal Key Clinical Specialty(shslczdzk06601) Biomaterials and Regenerative Medicine Institute Cooperative Research Project,Shanghai Jiao Tong University School of Medicine(2022LHA07) 

主　　题：JNK inhibitor chondrogenic medium in vitro in vivo cartilage regeneration 

摘      要：Cartilage tissue engineering is a promising strategy for repairing cartilage ***,achieving satisfactory cartilage regeneration in vitro and maintaining its stability in vivo remains a *** key to achieving this goal is establishing an efficient cartilage regeneration culture system to retain sufficient active cells with physiological functions,generate abundant cartilage extracellular matrix(ECM)and maintain a low level of cartilage ECM *** current chondrogenic medium(CM)can effectively promote cartilage ECM production;however,it has a negative effect on cell ***,the specific c-Jun N-terminal kinase pathway inhibitor SP600125 promotes chondrocyte proliferation but inhibits ECM ***,we aimed to construct a three-dimensional cartilage regeneration model using a polyglycolic acid/polylactic acid scaffold in combination with chondrocytes to investigate the effect of different culture modes with CM and SP600125 on in vitro cartilage regeneration and their long-term outcomes in vivo *** results demonstrate that the long-term combination of CM and SP600125 made up for each other and maximized their respective advantages to obtain optimal cartilage regeneration in ***,the long-term combination achieved stable cartilage regeneration after implantation in vivo with a relatively low initial cell-seeding ***,the long-term combination of CM and SP600125 enhanced in vitro and in vivo cartilage regeneration stability with fewer initial seeding cells and thus optimized the cartilage regeneration culture system.