The parallel biosynthesis routes of hyperoside from naringenin in Hypericum monogynum
作者机构：Jiangsu Key Laboratory of Bioactive Natural Product Research and State Key Laboratory of Natural MedicinesDepartment of Traditional Chinese PharmacyChina Pharmaceutical UniversityNanjing 210009China
出 版 物：《Horticulture Research》 (园艺研究（英文）)
年 卷 期：2023年第10卷第9期
页 面：247-259页
核心收录：
学科分类：09[农学] 0902[农学-园艺学] 090201[农学-果树学]
基 金：supported by the National Natural Science Foundation of China(No.32070389) the‘Double First-Class’University project of China Pharmaceutical University(CPU2022QZ29)
主 题：interior concerning hyper
摘 要：Hyperoside is a bioactive flavonoid galactoside in both medicinal and edible *** plays an important physiological role in the growth of flower ***,the hyperoside biosynthesis pathway has not been systematically elucidated in plants,including its original source,*** group found abundant hyperoside in the flower buds of Hypericum monogynum,and we sequenced its transcriptome to study the biosynthetic mechanism of *** gene screening and functional verification,four kinds of key enzymes were ***,HmF3Hs(flavanone 3-hydroxylases)and HmFLSs(flavonol synthases)could catalyze flavanones into dihydroflavonols,as well as catalyzing dihydroflavonols into *** could also convert flavanones into flavonols and flavones with varying ***3′H(flavonoid 3′-hydroxylase)was found to act broadly on 4′-hydroxyl flavonoids to produce 3′,4′-diydroxylated flavanones,dihydroflavonols,flavonols,and ***(flavonoid 3-O-galactosyltransferase)would transform flavonols into the corresponding 3-O-galactosides,including *** parallel hyperoside biosynthesis routes were thus depicted,one of which was successfully reconstructed in Escherichia coli BL21(DE3)by feeding naringenin,resulting in a hyperoside yield of 25 mg/***,this research not only helped us understand the interior catalytic mechanism of hyperoside in *** concerning flower development and bioactivity,but also provided valuable insights into these enzyme families.
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