Development of photoreactive demineralized bone matrix 3D printing colloidal inks for bone tissue engineering

作     者：Katie J.Hogan Hayriye Oztatlı Marissa R.Perez Sophia Si Reyhan Umurhan Elysa Jui Ziwen Wang Emily Y.Jiang Sa R.Han Mani Diba K.Jane Grande-Allen Bora Garipcan Antonios G.Mikos 

作者机构：Department of BioengineeringRice UniversityMS-1426500 Main StreetHoustonTX 77030USA Baylor College of Medicine Medical Scientist Training ProgramHoustonTX 77030USA Institute of Biomedical EngineeringBo�gazic¸i University_Istanbul34684Turkey 

出 版 物：《Regenerative Biomaterials》 (再生生物材料（英文版）)

年 卷 期：2023年第10卷第1期

页      面：1604-1617页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 0710[理学-生物学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 10[医学] 

基　　金：support from a National Science Foundation Graduate Research Fellowship(M.R.P.,E.J.,E.Y.J.) the National Institutes of Health(F31 DE030333,K.J.H P41 EB023833,A.G.M.) the Baylor College of Medicine Medical Science Training Program(K.J.H.),the Scientific and Technological Research Council of Turkey International Research Fellowship Programme for PhD Students(H.O.) a Rubicon Postdoctoral Fellowship from the Dutch Research Council(NWO Project No.019.182 EN.004)(M.D.) 

主　　题：demineralized bone matrix colloidal hydrogels 3D printing bone tissue engineering 

摘      要：Demineralized bone matrix(DBM)has been widely used clinically for dental,craniofacial and skeletal bone repair,as an osteoinductive and osteoconductive material.3D printing(3DP)enables the creation of bone tissue engineering scaffolds with complex geometries and *** methacryloylated gelatin nanoparticles(GNP-MAs)3DP inks have been developed,which display gel-like behavior for high print fidelity and are capable of post-printing photocrosslinking for control of scaffold swelling and ***,novel DBM nanoparticles(DBM-NPs,∼400 nm)were fabricated and characterized prior to incorporation in 3DP *** objectives of this study were to determine how these DBM-NPs would influence the printability of composite colloidal 3DP inks,assess the impact of ultraviolet(UV)crosslinking on 3DP scaffold swelling and degradation and evaluate the osteogenic potential of DBM-NP-containing composite colloidal *** addition of methacryloylated DBM-NPs(DBM-NP-MAs)to composite colloidal inks(100:0,95:5 and 75:25 GNP-MA:DBM-NP-MA)did not significantly impact the rheological properties associated with printability,such as viscosity and shear recovery or *** crosslinking with a UV dosage of 3 J/cm2 directly impacted the rate of 3DP scaffold swelling for all GNP-MA:DBM-NP-MA ratios with an∼40%greater increase in scaffold area and pore area in uncrosslinked versus photocrosslinked scaffolds over 21 days in phosphate-buffered saline(PBS).Likewise,degradation(hydrolytic and enzymatic)over 21 days for all DBM-NP-MA content groups was significantly decreased,∼45%less in PBS and collagenase-containing PBS,in UV-crosslinked versus uncrosslinked *** incorporation of DBM-NP-MAs into scaffolds decreased mass loss compared to GNP-MA-only scaffolds during collagenase *** in vitro osteogenic study with bone marrow-derived mesenchymal stem cells demonstrated osteoconductive properties of 3DP scaffolds for the DBM-NP-MA contents examine