Biocatalytic Buoyancy‑Driven Nanobots for Autonomous Cell Recognition and Enrichment

作     者：Ziyi Guo Chenchen Zhuang Yihang Song Joel Yong Yi Li Zhong Guo Biao Kong John MWhitelock Joseph Wang Kang Liang Ziyi Guo;Chenchen Zhuang;Yihang Song;Joel Yong;Yi Li;Zhong Guo;Biao Kong;John M.Whitelock;Joseph Wang;Kang Liang

作者机构：School of Chemical EngineeringAustralian Centre for NanoMedicineThe University of New South WalesSydneyNSW 2052Australia Medical CollegeNorthwest Minzu UniversityLanzhou 730000People’s Republic of China General Intensive Care UnitSecond Affiliated Hospital of Zhejiang University School of MedicineHangzhouPeople’s Republic of China School/Hospital of StomatologyLanzhou UniversityLanzhou 730000People’s Republic of China Department of ChemistryShanghai Key Lab of Molecular Catalysis and Innovative MaterialsCollaborative Innovation Center of Chemistry for Energy MaterialsFudan UniversityShanghai 200438People’s Republic of China Graduate School of Biomedical EngineeringThe University of New South WalesSydneyNSW 2052Australia Department of NanoengineeringUniversity of California San DiegoLa JollaCA 92093USA 

出 版 物：《Nano-Micro Letters》 (纳微快报（英文版）)

年 卷 期：2023年第15卷第12期

页      面：334-348页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 080202[工学-机械电子工程] 070205[理学-凝聚态物理] 0802[工学-机械工程] 0702[理学-物理学] 0713[理学-生态学] 

基　　金：supported by the Australian Research Council (ARC, DP210100422) the National Breast Cancer Foundation, Australia (IIRS-22-104) the financial support by the Australian Government Research Training Program Scholarship 

主　　题：Nanobots Surface functionalization Cell recognition Cell separation Metal-organic frameworks 

摘      要：Autonomously self-propelled nanoswimmers represent the nextgeneration nano-devices for bio-and environmental technology.However,current nanoswimmers generate limited energy output and can only move in short distances and duration,thus are struggling to be applied in practical challenges,such as living cell transportation.Here,we describe the construction of biodegradable metal-organic framework based nanobots with chemically driven buoyancy to achieve highly efficient,long-distance,directional vertical motion to“find-and-fetchtarget cells.Nanobots surface-functionalized with antibodies against the cell surface marker carcinoembryonic antigen are exploited to impart the nanobots with specific cell targeting capacity to recognize and separate cancer cells.We demonstrate that the self-propelled motility of the nanobots can sufficiently transport the recognized cells autonomously,and the separated cells can be easily collected with a customized glass column,and finally regain their full metabolic potential after the separation.The utilization of nanobots with easy synthetic pathway shows considerable promise in cell recognition,separation,and enrichment.