Mannitol inhibits the proliferation of neural stem cell by a p38 mitogen-activated protein kinase-dependent signaling pathway

作     者：Hai-Zhen Duan Xin Zhou Quan Hu Meng-Long Liu Shu-Hong Wang Ji Zhang Xu-Heng Jiang Tian-Xi Zhang An-Yong Yu Hai-Zhen Duan;Xin Zhou;Quan Hu;Meng-Long Liu;Shu-Hong Wang;Ji Zhang;Xu-Heng Jiang;Tian-Xi Zhang;An-Yong Yu

作者机构：Department of EmergencyAffiliated Hospital of Zunyi Medical UniversityZunyi563000Guizhou provinceChina Dazhou Vocational College of Chinese MedicineDazhou635000Sichuan provinceChina Dachuan District Traditional Chinese Medicine HospitalDazhou635000Sichuan provinceChina 

出 版 物：《Chinese Journal of Traumatology》 (中华创伤杂志（英文版）)

年 卷 期：2024年第27卷第1期

页      面：42-52页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：National Natural Science Foundation of China(no.82260385 and 82260254) Health commission of Guizhou Province(gzwkj2022-103) Chinese Ministry of Education(no.2020-39) Science and Technology Project of Guizhou province(no.20204Y149 and 2023580) 

主　　题：Mannitol Cerebral edema Neural stem cell Proliferation AQP4 p38 MAPK signaling pathway 

摘      要：Purpose:Mannitol is one of the first-line drugs for reducing cerebral edema through increasing the extracellular osmotic ***,long-term administration of mannitol in the treatment of cerebral edema triggers damage to neurons and *** that neural stem cell(NSC)is a subpopulation of main regenerative cells in the central nervous system after injury,the effect of mannitol on NSC is still *** present study aims to elucidate the role of mannitol in NSC ***:C57 mice were derived from the animal house of Zunyi Medical University.A total of 15 pregnant mice were employed for the purpose of isolating NSCs in this ***,mouse primary NSCs were isolated from the embryonic cortex of mice and subsequently identified through immunofluorescence *** order to investigate the impact of mannitol on NSC proliferation,both cell counting kit-8 assays and neurospheres formation assays were *** vitro effects of mannitol were examined at various doses and time *** order to elucidate the role of Aquaporin 4(AQP4)in the suppressive effect of mannitol on NSC proliferation,various assays including reverse transcription polymerase chain reaction,western blotting,and immunocytochemistry were conducted on control and mannitol-treated ***,the phosphorylated p38(p-p38)was examined to explore the potential mechanism underlying the inhibitory effect of mannitol on NSC ***,to further confirm the involvement of the p38 mitogen-activated protein kinase-dependent(MAPK)signaling pathway in the observed inhibition of NSC proliferation by mannitol,SB203580 was *** data were analyzed using SPSS 20.0 software(SPSS,Inc.,Chicago,IL).The statistical analysis among multiple comparisons was performed using one-way analysis of variance(ANOVA),followed by Turkey’’s post hoc test in case of the data following a normal distribution using a Shapiro-Wilk normality *** betwe