Aconite aqueous extract inhibits the growth of hepatocellular carcinoma through CCL2-dependent enhancement of natural killer cell infiltration

作     者：Kang-di Yang Xu Zhang Ming-cong Shao Li-na Wang Kang-di Yang;Xu Zhang;Ming-cong Shao;Li-na Wang

作者机构：Department of Traditional Chinese MedicineChanghai HospitalNaval Medical UniversityShanghai 200433China 

出 版 物：《Journal of Integrative Medicine》 (结合医学学报（英文版）)

年 卷 期：2023年第21卷第6期

页      面：575-583页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：funded by the National Natural Science Foundation of China(No.81774244) 

主　　题：Aconite Natural killer cell Tumor infiltration Chemokine CC chemokine ligand 2 Hepatoma 

摘      要：Objective:Aconite is a traditional Chinese herbal medicine that has been found to inhibit the development of liver cancer;however,its exact molecular mechanisms in this process remain *** study explores how aconite aqueous extract(AAE)inhibits hepatocellular carcinoma(HCC).Methods:An in vivo mouse model of subcutaneous liver cancer was *** AAE treatment,immunohistochemistry(IHC)was used to determine the effect of AAE on natural killer(NK)***,C57BL/6 mice were used to establish the subcutaneous tumor model,and a group of these mice were treated with anti-PK163 antibody to remove NK cells,which was verified by flow cytometry and *** effect of AAE on the proliferation of HCC cells in vitro was determined using cell counting *** effect of AAE on chemokine production in HCC cells was measured using real-time quantitative polymerase chain reaction and an enzyme-linked immunosorbent *** effect of AAE on the migration of NK cells was determined using a transwell ***,the molecular mechanism was investigated using the Western blotting ***:We demonstrated that the ability of AAE to induce overexpression of the cytokine C–C motif chemokine ligand 2(CCL2)in HCC cells is fundamental to the infiltration of NK cells into the tumor ***,we found that the upregulation of CCL2 was achieved by the activation of c-Jun Nterminal kinase but not extracellular regulated protein kinase or ***:Our findings suggest that AAE can be used as an effective immune adjuvant to enhance antitumor immunity by increasing NK cell infiltration into tumors,which could help to improve the efficacy of HCC treatments.