Histone H3K27me3 methylation regulates the expression of secreted proteins distributed at fast-evolving regions through transcriptional repression of transposable elements

作     者：XIE Jia-hui TANG Wei LU Guo-dong HONG Yong-he ZHONG Zhen-hui WANG Zong-hua ZHENG Hua-kun XIE Jia-hui;TANG Wei;LU Guo-dong;HONG Yong-he;ZHONG Zhen-hui;WANG Zong-hua;ZHENG Hua-kun

作者机构：State Key Laboratory of Ecological Pest Control for Fujian and Taiwan CropsCollege of Plant ProtectionFujian Agriculture and Forestry UniversityFuzhou 350002P.R.China Fujian Universities Key Laboratory for Plant Microbe InteractionCollege of Life SciencesFujian Agriculture and Forestry UniversityFuzhou 350002P.R.China Fuzhou Institute of OceanographyMinjiang UniversityFuzhou 350108P.R.China National Engineering Research Center of JUNCAO TechnologyCollege of Life ScienceFujian Agriculture and Forestry UniversityFuzhou 350002P.R.China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2023年第22卷第10期

页      面：3059-3068页

核心收录：

学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 0901[农学-作物学] 

基　　金：the grants from the National Natural Science Foundation of China(U1805232,31770156,and 32172365) the China Postdoctoral Science Foundation(2021M690637)。 

主　　题：secreted protein transposable elements fast-evolving regions H3K27me3 

摘      要：The fine-tuned expression dynamics of the effector genes are pivotal for the transition from vegetative growth to host colonization of pathogenic filamentous fungi.However,mechanisms underlying the dynamic regulation of these genes remain largely unknown.Here,through comparative transcriptome and chromatin immunoprecipitation sequencing(ChIP-seq)analyses of the methyltransferase PoKmt6 in rice blast fungus Pyricularia oryzae(syn.Magnaporthe oryzae),we found that PoKmt6-mediated H3K27me3 deposition was enriched mainly at fast-evolving regions and contributed to the silencing of a subset of secreted proteins(SP)and transposable element(TE)families during the vegetative growth of P.oryzae.Intriguingly,we observed that a group of SP genes,which were depleted of H3K27me3 modification,could also be silenced via the H3K27me3-mediated repression of the nearby TEs.In conclusion,our results indicate that H3K27me3 modification mediated by PoKmt6 regulates the expression of some SP genes in fast-evolving regions through the suppression of nearby TEs.