Cr（V）involvementin the toxicity pathway of testicular damage

作     者：M.L.Pereira T.M.Santos 等 

作者机构：DepartmentofBiology DepartmentofchemistryUniversityofAveriro3810－193AveiroPortugal 

出 版 物：《Asian Journal of Andrology》 (亚洲男性学杂志（英文版）)

年 卷 期：2002年第4卷第2期

页      面：153-155页

核心收录：

学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学] 

主　　题：Blood-testis barrier Cr(V) Horseradish peroxidase Integrity Spermatogenesis 

摘      要：OBJECTIVE:The functional integrity of the blood-testis barrier (BTB) in male mice exposed to Cr(V) was studied in order to clarify the mechanism underlying testicular injury.;METHODS:Adult male mice were subcutaneously injected repeated doses of 8.02 micromol (0.5 ml) of Cr/*** for 5 days. Animals receiving a similar volume of bis(hydroxyethyl)-aminotris(hydroxymethyl)methane buffer (BT) were used as controls. The animals were sacrificed on day 6 and small fragments of seminiferous tubules, approximately 8-10 mm length, were incised and sutured at both ends. They were exposed in vitro to horseradish peroxidase-containing culture medium for 10 minutes. Tissues were then fixed and processed for ultrastructural studies.;RESULTS:Controls and Cr(V)-treated group resulted in the uptake of the tracer by Sertoli cells. However, the major finding consisted in the permeability of the BTB only in the Cr(V)-group, as evidenced by the presence of the tracer within the junctions between the neighbouring Sertoli cells.;CONCLUSIONS:The BTB is disrupted in mice submitted to Cr(V). The permeability of the BTB is a crucial feature to be investigated for the understanding of lesions within the seminiferous tubule.