Molecular Discrimination of Wild Philippine Paddy Straw Mushroom （ Volvariella volvacea）
Molecular Discrimination of Wild Philippine Paddy Straw Mushroom （ Volvariella volvacea）作者机构：Department of Biological Sciences College of Arts and Sciences Central Luzon State University Science City of Munoz Nueva Ecija 3120 Philippines Faculty of Life Sciences The University of Manchester United Kingdom Oxford Rd. M13 9PL UK
出 版 物：《Journal of Life Sciences》 (生命科学（英文版）)
年 卷 期：2014年第8卷第1期
页 面：27-34页
学科分类：09[农学] 0901[农学-作物学] 0902[农学-园艺学] 090202[农学-蔬菜学]
主 题：Volvariella volvacea genetic diversity random amplified polymorphic DNA (RAPD).
摘 要：Genetic diversity study of wild Philippine paddy straw mushroom （Volvariella volvacea） was conducted to establish a germplasm collection accessible to volvariella producers in the Philippines. Forty one wild strains were collected from different geographical areas in Northern and Central Luzon region. Strains were differentiated using random amplified polymorphic DNA （RAPD）. A single 10-based primer was used to generate randomly amplified polymorphic DNA （RAPD） in V. volvacea and differences were noted in band size （bp） ranging from 1,800 bp to 550 bp. Principal component analysis （PCA） of the RAPD data revealed 4 groups from wild strains. One strain showed RAPD pattern with band appearance at 1,750, 950 and 750 bp; 3 strains at 1,800 and 750 bp; 8 strains at 1,500 and 550; and the most abundant group with 29 strains at 750 bp. With observed lack of heterogeneity among strains, it is recommended that more collections from the wild should be undertaken for more diverse germplasm collection. Moreover, it is suggested that RAPD can be used to delineate strains of V. volvacea with potential importance on genetic diversity conservation and breeding.
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