Naringenin suppresses NLRP3 inflammasome activation via the mRNA-208a signaling pathway in isoproterenol-induced myocardial infarction

作     者：Ayman Eldourghamy Toka Hossam Mohammed Abdalla Hussein Amal Abdel-Aziz Samir A.El-masry Ayman Eldourghamy;Toka Hossam;Mohammed Abdalla Hussein;Amal Abdel-Aziz;Samir A.El-masry

作者机构：Environmental Biotechnology DepartmentGenetic Engineering and Biotechnology Research InstituteSadat UniversityEgypt Department of Medical LabsFaculty of Applied Medical Sciences TechnologyOctober 6 UniversityEgypt Department of BiotechnologyFaculty of Applied Health Sciences TechnologyOctober 6 UniversityEgypt Molecular Biology DepartmentGenetic Engineering and Biotechnology Research InstituteSadat UniversityEgypt 

出 版 物：《Asian Pacific Journal of Tropical Biomedicine》 (亚太热带生物医学杂志（英文版）)

年 卷 期：2023年第13卷第10期

页      面：443-450页

核心收录：

学科分类：1006[医学-中西医结合] 1002[医学-临床医学] 10[医学] 100602[医学-中西医结合临床] 

主　　题：Naringenin Isoproterenol Myocardial infarction Antioxidants NLRP3 mRNA-208a 

摘      要：Objective:To investigate the cardioprotective effect of naringenin against isoproterenol(ISO)-induced cardiotoxicity in rats.Methods:Rats were divided into five groups:the normal group,the ISO group(85 mg/kg b.w.);the ISO+naringenin(50 mg/kg b.w.)group,the ISO+naringenin(100 mg/kg b.w.)group and the ISO+propranolol(10 mg/kg b.w.)group.Plasma creatine kinase-MB(CK-MB),cardiac troponin T,lactate dehydrogenase,brain natriuretic peptide(BNP),and IL-10,as well as cardiac transforming growth factor-β1(TGF-β1),vascular endothelial growth factor(VEGF)and malondialdehyde(MDA)were examined.In addition,NLRP3 and mRNA-208a expressions were evaluated by RT-PCR analysis.Histopathological examination was also performed to assess cardiac damages.Results:Naringenin treatment significantly decreased plasma lactate dehydrogenase,CK-MB,cardiac troponin T,BNP,and IL-10,as well as cardiac TGF-β1,VEGF,and MDA while increasing p-Akt and superoxide dismutase in ISO-administered rats.It also reduced NLRP3 and mRNA-208a gene expression levels.Furthermore,naringenin improved ISO-induced cardiac damage.Conclusions:Naringenin attenuates myocardial dysfunction in ISO-treated rats by decreasing oxidative stress and increasing cardiac endogenous antioxidant system,which may be modulated partly by improvement of NLRP3 and mRNA-208a gene expression.