Methylation in hangtaimycin biosynthesis and its antibacterial activities
作者机构：School of Pharmacy and BioengineeringChongqing University of TechnologyChongqingChina Key Laboratory of Combinatorial Biosynthesis and Drug DiscoveryMinistry of Educationand School of Pharmaceutical SciencesWuhan UniversityWuhanChina
出 版 物：《Synthetic and Systems Biotechnology》 (合成和系统生物技术（英文）)
年 卷 期：2023年第8卷第4期
页 面：682-687页
核心收录：
学科分类：1007[医学-药学(可授医学、理学学位)] 10[医学]
基 金：supported by National Key R&D Program of China(2018YFA0903200) General Program of National Natural Science Foundation of China(32370083) Natural Science Foundation of Chongqing CSTB(CSTB2022NSCQ-MSX0995) Graduate Innovation Program(GZLCX20232114)
主 题：Hangtaimycin Methylation S-Adenosylmethionine-dependent methyltransferases FK506 methyltransferase type O-methyltransferase Antibacterial Biosynthesis
摘 要：About two-thirds of small molecule drugs contain methyl group and it plays a very important role in the drug ***,methyltransferases catalyzing the methylation have always attracted great ***(HTM)is a potent hepatoprotective *** study showed that its biosynthetic gene cluster contained three methyltransferase domains,but their characteristics in HTM biosynthetic pathway has not been *** this study,we clarified multi-methylations in HTM biosynthesis in *** showed that the two S-adenosylmethionine-dependent methyltransferases(SAM-MTs)of HtmA2(-module 6)-MT domain and HtmB2(-module 18)-MT domain are responsible for the installation of methyl group at C-45 and N-12,respectively,whereas the FK506 methyltransferase(FKMT)type O-methyltransferase of HtmB1(-module 16)-MT domain take care of the methylation at O-21 of *** also reported the antibacterial activities of HTM in this study,and found that it showed activities against ***,*** and *** with MIC of 4μg/mL,4μg/mL,and 64μg/mL,respectively.
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