Microfluidic magnetic detection system combined with a DNA framework-mediated immune-sandwich assay for rapid and sensitive detection of tumor-derived exosomes
作者机构：National Key Laboratory of Materials for Integrated CircuitsShanghai Institute of Microsystem and information TechnologyChinese Academy of Sciences865 Changning RoadShanghai 200050China Shanghai Advanced Research InstituteChinese Academy of SciencesShanghai 201210China University of Chinese Academy of SciencesBejing 100049China school of Information Science and TechnologyShanghai Tech UniversityShanghai 201210China School of Life SciencesShanghai UniversityShanghai 20044China School of Physics and Optoelectronic Engineering Hangzhou Institute for Advanced StudyUniversity of Chinese Academy of SciencesChinese Academy of SciencesHangzhou 310024China
出 版 物：《Microsystems & Nanoengineering》 (微系统与纳米工程（英文）)
年 卷 期：2023年第9卷第6期
页 面：195-204页
核心收录：
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金：funded by the Program of Shanghai Academic/Technology Research Leader,grant number 20xD 1404600 Shanghai Municipal Science and Technology Commission,grant numbers 20511107600 and 19511107102 Chinese Academy of Science,grant number KFJ-STS-QYZD-20210-08-002 National Key Research and Development Program of China,grant number 2016YFC0100600
摘 要：Tumor-derived circulating exosomes(TDEs)are being pursued as informative and noninvasive ***,quantitatively detecting TDEs is still ***,we constructed a DNA tetrahedral-structured probe(TSP)-mediated microfluidic magnetic detection system(μFMS)to provide a rapid and sensitive platform for analyzing ***63 aptamer-modified Fe_(3)O_(4)magnetic nanoparticles(MNPs)were constructed to form magnetic nano-report probes(MNRs).The microfluidic chips were fabricated from glass functionalized with DNA TSP-modified aldehyde groups and a PDMS layer designed with serpentine *** induction coil-based magnetic detector was used to measure the magnetic *** linear dynamic range of theμFMS system for TDE assays was 1.98×10^(3)-1.98×10^(7)particles/mL with a limit of detection of 1.98×10^(3)particles/mL in *** was no significant difference in TDE detection between the simulated serum and PBS,which indicated the feasibility of the constructedμFMS system for TDE analysis in complex biological *** terms of cost,reaction time and operation procedure,thisμFMS has the potential to be developed as a clinical point-of-care testing tool for cancer diagnosis and therapeutics.
维普期刊数据库