Essential procedures of single-cell RNA sequencing in multiple myeloma and its translational value

作     者：Jun Du Xiao-Ran Gu Xiao-Xiao Yu Yang-Jia Cao Jian Hou 

作者机构：Department of HematologyRenji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200127China School of MedicineShanghai Jiao Tong UniversityShanghai 200025China Department of HematologyFirst Affiliated Hospital of Xi’an Jiaotong UniversityXi’anShanxi 710000China 

出 版 物：《Blood Science》 (血液科学（英文）)

年 卷 期：2023年第5卷第4期

页      面：221-236页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported by the Shanghai Shenkang Hospital Development Center(No.SHDC2020CR2070B) 

主　　题：Evolution Microenvironment Multiple myeloma scRNA-seq Target therapy 

摘      要：Multiple myeloma(MM)is a malignant neoplasm characterized by clonal proliferation of abnormal plasma *** many countries,it ranks as the second most prevalent malignant neoplasm of the hematopoietic *** treatment methods for MM have been continuously improved and the survival of patients has been dramatically prolonged,MM remains an incurable disease with a high probability of *** such,there are still many challenges to be *** promising approach is singlecell RNA sequencing(scRNA-seq),which can elucidate the transcriptome heterogeneity of individual cells and reveal previously unknown cell types or states in complex *** this review,we outlined the experimental workflow of scRNA-seq in MM,listed some commonly used scRNA-seq platforms and analytical *** addition,with the advent of scRNA-seq,many studies have made new progress in the key molecular mechanisms during MM clonal evolution,cell interactions and molecular regulation in the microenvironment,and drug resistance mechanisms in target *** summarized the main findings and sequencing platforms for applying scRNA-seq to MM research and proposed broad directions for targeted therapies based on these findings.