Thymol ameliorates ammonium toxicity via repressing polyamine oxidase-derived hydrogen peroxide and modulating ammonium transporters in rice root

作     者：Kai Guo Guangchi An Ning Wang Bingdong Pang Zhiqi Shi Hongwu Bai Li Zhang Jian Chen Weimin Xu 

作者机构：National Tobacco Cultivation and Physiology and Biochemistry Research Center/Key Laboratory for Tobacco Cultivation of Tobacco IndustryHenan Agricultural UniversityZhengzhou 450002China Biology InstituteQilu University of Technology(Shandong Academy of Sciences)Shandong Province Key Laboratory for BiosensorsJinan 250014China Zaozhuang Technology Promotion Center of Agriculture and Agricultural MachineryZaozhuang 277800China Central LaboratoryJiangsu Academy of Agricultural SciencesNanjing 210014China Institute of Food Safety and NutritionJiangsu Academy of Agricultural Sciences/Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and TechnologyNanjing 210014China College of Plant ProtectionShandong Agricultural UniversityTaian 271018China Supervision&Testing Center for Processed Agri-products(Nanjing)Ministry of AgricultureNanjing 210014China 

出 版 物：《Food Production, Processing and Nutrition》 (食物生产加工与营养(英文))

年 卷 期：2021年第3卷第1期

页      面：106-114页

核心收录：

学科分类：0832[工学-食品科学与工程（可授工学、农学学位）] 09[农学] 0901[农学-作物学] 

基　　金：Central Government Guides Local Science and Technology Development Fund Projects, (YDZX20203700003919) Program for Youth Innovation and Technology Team in University of Shandong Province, (13, 2019KJF016) Science and Technology Project of Yantai, (2019ZDCX016) Shandong Academy of Science Fund for Distinguished Young Scholars, (LKYZ2018-60) Special Science and Technology Project of Rizhao, (2019CXZX2205) State Key Laboratory of Plant Cell and Chromosome Engineering, (PCCE-KF-2019-05) National Natural Science Foundation of China, NSFC, (31771705) China Postdoctoral Science Foundation, (2015 M570627) 

主　　题：Ammonium toxicity Polyamine oxidase Reactive oxygen species Rice Thymol 

摘      要：Background:Ammonium is an indispensable nutrient for crop growth,but anoxic conditions or inappropriate fertilizer usage result in the increase in ammonium content in soil.Excessive ammonium causes phytotoxicity.Thymol is a kind of natural phenolic compound with anti-microbial properties.However,little is known about the role of thymol in modulating plant physiology.Here we find the novel role of thymol in protecting rice from ammonium toxicity.Results:Thymol remarkably rescued rice seedlings growth from ammonium stress,which may resulted from the attenuation of reactive oxygen species(ROS)accumulation,oxidative injury,and cell death in both shoots and roots.Polyamine oxidase(PAO)metabolizes polyamines to produce ROS in plants in response to stress conditions.Thymol blocked ammonium-induced upregulation of a set of rice PAOs,which contributed to the decrease in ROS content.In rice seedlings upon ammonium stress,thymol downregulate the expression of ammonium transporters(AMT1;1 and AMT1;2);thymol upregulated the expression of calcineurin B-like interacting protein kinase 23(CIPK23)and calcineurin B-like binding protein 1(CBL1),two negative regulators of AMTs.This may help rice avoid ammonium overload in excessive ammonium environment.Correlation analysis indicated that PAOs,AMTs,and CBL1 were the targets of thymol in the detoxification of excessive ammonium.Conclusion:Thymol facilitates rice tolerance against ammonium toxicity by decreasing PAO-derived ROS and modulating ammonium transporters.Such findings may be applicable in the modulation of nutrient acquisition during crop production.