Functional genomic studies on an immune-and antiviral-related gene of MyD88 in orange-spotted grouper,Epinephelus coioides
Functional genomic studies on an immune-and antiviral-related gene of MyD88 in orange-spotted grouper,Epinephelus coioides作者机构：Key Laboratory of Marine Bio-resources Sustainable UtilizationSouth China Sea Institute of OceanologyChinese Academy of SciencesGuangzhou 510301China State Key Laboratory of Biocontrol School of Life SciencesSun Yat-sen UniversityGuangzhou 510275China
出 版 物：《Chinese Science Bulletin》 (中国科学通报)
年 卷 期：2012年第57卷第25期
页 面：3277-3287页
核心收录：
基 金：supported by the National Basic Research Program of China (2012CB114402) the Knowledge Innovation Program of the Chinese Academy of Sciences (KZCX2-YW-BR-08 and KZCX2- EW-Q213) the National Natural Science Foundation of China (30725027 and 30930070)
主 题：抗病毒相关基因 功能基因组学 石斑鱼 免疫相关 Toll样受体 核因子-κB 氨基酸序列 死亡结构域
摘 要：Myeloid differentiation factor 88(MyD88) is a universal adaptor protein involved in Toll-like receptors and in interleukin-1 receptor-induced nuclear factor-kappaB(NF-κB) *** this study,a new MyD88 gene(designated as Og-MyD88) was cloned from orange-spotted grouper,Epinephelus coioides,based on the expressed sequence tag(EST) obtained following Roche 454 GS-FLX *** full-length Og-MyD88 cDNA is composed of 1682 bp and encodes a deduced polypeptide of 289 amino acids with 86% homology to MyD88 of Siniperca *** deduced amino acid sequence of Og-MyD88 contains a typical death domain at the amino terminus and a conserved Toll/IL-1R(TIR) domain at the carboxyl terminus,as well as three highly conserved motifs(Box1,Box2 and Box3) within the C-terminal TIR *** healthy fish,Og-MyD88 was found to be strongly expressed in immune-related tissues,including the spleen,head kidney,kidney,liver,skin and intestine,with lower expression in heart,stomach,brain and *** of Og-MyD88 were found to be markedly up-regulated in fish spleen after challenge with Singapore grouper iridovirus(SGIV),a highly lethal viral pathogen to grouper ***,the full length Og-MyD88 and its N-terminal death domain were capable of inducing NF-κB activity in HEK-293 *** Og-MyD88 showed the ability to inhibit replication of SGIV in grouper spleen(GS) *** results suggest that Og-MyD88 is involved in the grouper immune response to invasion of viral pathogens and may share similar functions to those observed in higher vertebrates.
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