Autophagy mediates osteoporotic bone regeneration induced by micro-/nano-structured modification on hydroxyapatite bioceramics

作     者：Jinjie Cui Xinran Zhang Liming Cheng Kaili Lin 

作者机构：Department of Oral and Cranio-maxillofacial SurgeryCollege of StomatologyNational Center for StomatologyNational Clinical Research Center for Oral DiseasesShanghai Key Laboratory of StomatologyShanghai Research Institute of StomatologyShanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineShanghai Jiao Tong UniversityShanghai 200011China Shanghai Engineering Research Center of Tooth Restoration and RegenerationSchool and Hospital of StomatologyTongji UniversityShanghai 200072China Department of Spine SurgeryTongji HospitalTongji University School of MedicineShanghai 200065China Ministry of EducationKey Laboratory of spine and spinal cord injury repair and regeneration(Tongji University)ShanghaiChina 

出 版 物：《再生工程(英文)》 (Engineered Regeneration)

年 卷 期：2023年第4卷第3期

页      面：245-256页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 08[工学] 

基　　金：support from the National Natural Science Foundation of China (82072396,32271379) Science and Technology Commission of Shanghai Municipality (21490711700) the Interdisciplinary Program of Shanghai Jiao Tong University (YG2021ZD12). 

主　　题：Osteoporosis Osteogenesis Autophagy Hydroxyapatite Micro-/nano-structured modification 

摘      要：Osteoporosis(OP)is an age-related disease of bone metabolism,characterized by bone mass loss and bone mi-croarchitecture deterioration,the poor osteogenesis microenvironment of OP caused hardly repairing of the bone defects.As a dynamic process to fuel cellular renovation,autophagy has been proved to play a vital role in regulating cell differentiation and maintaining bone homeostasis.Traditional bone repairing biomaterials are hardly repairing the bone defects under OP pathological microenvironment.Therefore,it is essential to develop-ment novel biomaterials to improve osteoporotic osteogenesis.Compared to biochemical cues,biophysical cues exhibited more advantages in biocompatible and side effects.Herein,inspired by the importance of enhanced au-tophagic response in osteoporotic environment,we intend to utilize the micro-/nano-structured hydroxyapatite(mnHA)bioceramics as the mimic structure of natural bone tissue to regulate autophagic activity in ovariectomy bone mesenchymal stem cells(OVX-BMSCs),finally promote to bone regeneration in OP condition.The results indicated that mnHA bioceramics promoted cell adhesion and osteogenesis of OVX-BMSCs,and enhanced au-tophagy level in OVX-BMSCs.In the calvarial defects of OVX-rats,the mnHA scaffold acquired excellent bone repair effect.According to the current findings,regulating the level of autophagy could be a promising strategy for improve osteoporotic osteogenesis in the future.