Label-free quantitative proteomics analysis models in vivo and in vitro reveal key proteins and potential roles in sciatic nerve injury

作     者：YANG GU MINGGUANG BI DEHUI CHEN NING NI JIANMING CHEN 

作者机构：Department of Trauma Orthopedics SurgeryNingbo No.6 HospitalNingbo University School of MedicineNingbo315000China Department of OrthopedicsNingbo Medical CenterLihuili HospitalNingbo University School of MedicineNingbo315000China Department of Orthopedic SurgeryFujian Medical University Union HospitalFuzhou350001China School of MedicineNingbo UniversityNingbo315000China 

出 版 物：《生物细胞(英文)》 (BIOCELL)

年 卷 期：2023年第47卷第9期

页      面：2069-2080页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学] 

基　　金：funded by the Project of 2022 Health Commission Technology Plan of Zhejiang Province(Project Number:2022KY1169) the 2022 Ningbo Natural Science Foundation Young Doctoral Project(Project Number:2022J027) 

主　　题：Oxygen glucose deprivation Proteomics Sciatic nerve injury Peripheral nerve injury 

摘      要：Background:The underlying mechanism of sciatic nerve injury(SNI)is a common motor functional disorder,necessitates further ***:A rat model of SNI was established,with the injury group subjected to compressive injury of the right sciatic nerve exposed at the midpoint of the thigh and the sham surgery group undergoing the same surgical *** oxygen-glucose deprivation model was employed to simulate in vitro SNI in PC12 *** data acquisition and quality control,differentially expressed proteins(DEPs)in each model were identified through differential analysis,and enrichment analysis was used to explore the potential functions and pathways of the *** diagrams were drawn,and DEPs from both in vivo and in vitro SNI models were imported into the STRING database to construct a protein-protein interaction network and screen for hub ***:After the peptide segments obtained from rat nerve blockade and PC12 cells met quality requirements,258 DEPs were identified in rat nerve samples,and 119 DEPs were screened in PC12 *** analysis revealed that DEPs in the rat model were predominantly concentrated in biological functions such as myogenic cell proliferation and signaling related to lipid and energy *** in the in vitro model were mainly enriched in biological processes such as phagocytosis and were associated with lipid transport and *** hub proteins,amyloid precursor protein(APP)and fibronectin 1(FN1),were identified through MCC,MCODE,and Degree *** PC12 cells and external validation sets showed relatively higher expression of APP and FN1 in injured *** of gene set enrichment analysis indicated that these two proteins were associated with metabolic pathways,such as biosynthesis of glycosaminoglycan chondroitin sulfate and biosynthesis of unsaturated fatty ***:APP and FN1 are potential key molecules involved in SNI and are associated with various metabolic pathways