Selenium deficiency-induced multiple tissue damage with dysregulation of immune and redox homeostasis in broiler chicks under heat stress

作     者：Ling Zhao Meng Liu Hua Sun Jia-Cheng Yang Yu-Xuan Huang Jia-Qiang Huang Xingen Lei Lv-Hui Sun 

作者机构：State Key Laboratory of Agricultural MicrobiologyHubei Hongshan LaboratoryFrontiers Science Center for Animal Breeding and Sustainable ProductionCollege of Animal Sciences and TechnologyHuazhong Agricultural UniversityWuhan 430070China Beijing Advanced Innovation Center for Food Nutrition and Human HealthDepartment of Nutrition and HealthChina Agricultural UniversityBeijing 100083China Department of Animal ScienceCornell UniversityIthaca 14853USA 

出 版 物：《中国科学:生命科学英文版》 (Science China: Life Sciences)

年 卷 期：2023年第66卷第9期

页      面：2056-2069页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China (32102588) the Top-notch Young Talent Supporting Program to LHS Beijing Deyuanshun Biological Technology Co.,Ltd。 

主　　题：selenium multiple tissue damage metabolome transcriptome broilers 

摘      要：Broiler chicks are fast-growing and susceptible to dietary selenium(Se) deficiency. This study sought to reveal the underlying mechanisms of how Se deficiency induces key organ dysfunctions in broilers. Day-old male chicks(n=6 cages/diet, 6 chicks/cage) were fed with a Se-deficient diet(Se-Def, 0.047 mg Se/kg) or the Se-Def+0.3 mg Se/kg(Control, 0.345 mg Se/kg) for 6weeks. The serum, liver, pancreas, spleen, heart, and pectoral muscle of the broilers were collected at week 6 to assay for Se concentration, histopathology, serum metabolome, and tissue transcriptome. Compared with the Control group, Se deficiency induced growth retardation and histopathological lesions and reduced Se concentration in the five organs. Integrated transcriptomics and metabolomics analysis revealed that dysregulation of immune and redox homeostasis related biological processes and pathways contributed to Se deficiency-induced multiple tissue damage in the broilers. Meanwhile, four metabolites in the serum, daidzein, epinephrine, L-aspartic acid and 5-hydroxyindoleacetic acid, interacted with differentially expressed genes with antioxidative effects and immunity among all the five organs, which contributed to the metabolic diseases induced by Se deficiency. Overall, this study systematically elucidated the underlying molecular mechanisms in the pathogenesis of Se deficiency-related diseases, which provides a better understanding of the significance of Se-mediated heath in animals.