Regulation of a vacuolar proton-pumping P-ATPase MdPH5 by MdMYB73 and its role in malate accumulation and vacuolar acidification
作者机构：National Research Center for Apple Engineering and TechnologyShandong Collaborative Innovation Center of Fruit and Vegetable Quality and Efficient ProductionCollege of Horticulture Science and EngineeringShandong Agricultural UniversityTai’an 271018ShandongChina
出 版 物：《aBIOTECH》 (生物技术通报（英文版）)
年 卷 期：2023年第4卷第4期
页 面：303-314页
核心收录：
学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1001[医学-基础医学(可授医学、理学学位)] 07[理学] 0901[农学-作物学] 0836[工学-生物工程] 0902[农学-园艺学] 070202[理学-粒子物理与原子核物理] 0702[理学-物理学]
基 金：supported by grants from the National Key Research and Development Program of China(2022YFD2100102) National Natural Science Foundation of China(32122080,31972375) Shandong Province(ZR2020YQ25)
主 题：Apple Malate accumulation P-ATPase MdPH5 MdMYB73
摘 要：As the main organic acid in fruits,malate is produced in the cytoplasm and is then transported into the *** accumulates by vacuolar proton pumps,transporters,and channels,affecting the taste and flavor of *** the three types of proton pumps(V-ATPases,V-PPases,and P-ATPases),the P-ATPases play an important role in the transport of malate into *** this study,the transcriptome data,collected at different stages after blooming and during storage,were analyzed and the results demonstrated that the expression of MdPH5,a vacuolar proton-pumping P-ATPase,was associated with both pre-and post-harvest malate ***,MdPH5 is localized at the tonoplast and regulates malate accumulation and vacuolar *** addition,MdMYB73,an upstream MYB transcription factor of MdPH5,directly binds to its promoter,thereby transcriptionally activating its expression and enhancing its *** this way,MdMYB73 can also affect malate accumulation and vacuolar ***,this study clarifies how MdMYB73 and MdPH5 act to regulate vacuolar malate transport systems,thereby affecting malate accumulation and vacuolar pH.
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