pIL-12 delivered by polymer based nanovector for anti-tumor genetherapy

作     者：Lianbin Wen Xin Zan Qidi Pang Yuzhu Hu Songping Zheng Mengni Ran Xiang Gao Xiang Wang Bilan Wang Lianbin Wen;Xin Zan;Qidi Pang;Yuzhu Hu;Songping Zheng;Mengni Ran;Xiang Gao;Xiang Wang;Bilan Wang

作者机构：Department of GeriatricsSichuan Academy of Medical Sciences&Sichuan Provincial People’s HospitalChengdu 610072China Department of NeurosurgeryWest China HospitalSichuan UniversityChengdu 610041China Department of Infection ManagementHospital of Chengdu University of Traditional Chinese MedicineChengdu 610072China Department of Radiation OncologyCancer CenterWest China HospitalWest China Medical SchoolSichuan UniversityChengdu 610041China Department of PharmacyWest China Second University Hospital of Sichuan UniversityChengdu 610041China Pharmaceutical DepartmentChongqing University Three Gorges HospitalChongqing 404000China 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2023年第34卷第11期

页      面：209-213页

核心收录：

学科分类：1002[医学-临床医学] 0703[理学-化学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China(No.81972347) the Key R&D Projects of the Science and Technology Department of Sichuan Province(No.2022YFS0324) 

主　　题：Non-viral vector Cancer gene therapy pIL-12 Immune response Nanocomposite 

摘      要：Finding more effective and safe non-viral vectors to transfer genes into cancer cells has become the key of immune gene therapy for *** a triblock compound MPEG_(2000)-PDLLA_(4000)-MPEG_(2000) modified by cationic liposome DOTAP was used as a non-viral vector DOTAP/MPEG_(2000)-PDLLA_(4000)-MPEG_(2000)(DMPM)to effectively transfer interleukin(IL)-12 plasmid(pIL-12)into tumor ***-12 produced by transfected tumor cells successfully inducing lymphocyte proliferation and promoting interferon-γ(IFN-γ)secretion,which resulted in tumor cells *** ability of DMPM to transfer pIL-12 and the immune effect induced by IL-12 in cells had been *** anti-tumor effect,mechanism and safety of pIL-12/DMPM in mice cancer model were investigated in this *** results showed that the pIL-12 transferred by DMPM was highly expressed both in CT26 cells and B16-F10 ***-12 expressed in the culture supernatant of transfected tumor cells stimulated lymphocyte proliferation and promoted IFN-γ*** experimental result confirmed that pIL-12/DMPM therapy significantly reduced tumor growth in mice *** designed the nanocomposite DMPM to deliver pIL-12 for cancer treatment and explored its therapeutic efficacy and the underlying anti-tumor *** study suggested pIL-12 loaded by DMPM complex would be an effective strategy for cancer treatment.