Carbonic anhydrase 2 mediates anti-obesity effects of black tea as thermogenic activator

作     者：Peng Ma Jie Xiao Biyu Hou Ping He Xinyu Yang Yisa Wang Zijing Wang Tianshu Xu Xiuying Yang Xuan Zhu Shasha Xiang Song Li Guanhua Du Jian Ying Guifen Qiang Peng Ma;Jie Xiao;Biyu Hou;Ping He;Xinyu Yang;Yisa Wang;Zijing Wang;Tianshu Xu;Xiuying Yang;Xuan Zhu;Shasha Xiang;Song Li;Guanhua Du;Jian Ying;Guifen Qiang

作者机构：State Key Laboratory of Bioactive Substance and Function of Natural MedicinesInstitute of Materia MedicaChinese Academy of Medical Sciences and Peking Union Medical College and Beijing Key Laboratory of Drug Target and Screening ResearchBeijing 100050China COFCO Nutrition and Health Research InstituteBeijing 102209China Department of PharmacyThe Eighth Affiliated HospitalSun Yat-sen UniversityShenzhen 518000China Faculty of BiologyMedicine and Health SciencesThe University of ManchesterManchester M139PLUnited Kingdom Changchun University of Chinese MedicineChangchun 130117China School of Food Science and BiotechnologyZhejiang Gongshang UniversityHangzhou 310018China China Tea Technology(Beijing)Co.Ltd.Beijing 102209China Beijing Key Laboratory of NutritionHealth and Food SafetyBeijing 102209China 

出 版 物：《Food Science and Human Wellness》 (食品科学与人类健康（英文）)

年 卷 期：2024年第13卷第5期

页      面：2917-2936页

核心收录：

学科分类：0832[工学-食品科学与工程（可授工学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1002[医学-临床医学] 08[工学] 09[农学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 090203[农学-茶学] 083201[工学-食品科学] 0902[农学-园艺学] 10[医学] 

基　　金：funded by National Natural Science Foundation of China(NSFC 82070877) CAMS Innovation Fund for Medical Sciences(CIFMS)(2022-I2M-JB-010,2021-I2M-1-005) The National High Technology Research and Development Program of China(2017YFE0112900) 

主　　题：Obesity Black tea Thermogenesis Browning Adipose tissue 

摘      要：Obesity is a metabolic disorder due to over-accumulation of adipose tissue and ultimately becomes a“disease.Brown adipose tissue(BAT)thermogenesis and white adipose tissue(WAT)browning emerge as a potential strategy of anti-obesity by dissipating energy as ***,drugs based on adipose tissue thermogenesis have not been successfully approved *** current study,we found that black tea extract(BTE)obtained by patentauthorized manufacturing process prevented body weight gain as novel thermogenic activator with reduction of adiposity,improvement of adipose distribution,and glucose metabolism improvement in diet-induced obesity ***,anti-obesity effect of BTE depends on promoting BAT thermogenesis and WAT browning with upregulation of uncoupling protein 1(UCP1),especially visceral adipose tissue(VAT)with browning ***,utilizing in silico approach of network pharmacology and molecular docking,we identified carbonic anhydrase 2(CA2)in nitrogen metabolism as anti-obesity target of BTE and further elucidated that protein kinase B(AKT)signaling pathway linked CA2 and *** gut microbiota regulation may prompt the CA2-dependent thermogenesis *** findings demonstrated anti-obesity effect of BTE as thermogenic activator through CA2-mediated BAT thermogenesis and WAT browning via CA2-AKT-UCP1 signaling pathway,which could be developed as promising anti-obesity agent with good safety and efficacy.