Anthocyanin-assisted Agrobacterium infiltration for the rapid evaluation of genome editing efficiencies across multiple plant species

作     者：Sinian Xing Yu Sun Boshu Li Hongchao Li Kevin T.Zhao Kunling Chen Caixia Gao Sinian Xing;Yu Sun;Boshu Li;Hongchao Li;Kevin T.Zhao;Kunling Chen;Caixia Gao

作者机构：State Key Laboratory of Plant Cell and Chromosome EngineeringCenter for Genome EditingInstitute of Genetics and Developmental BiologyInnovation Academy for Seed DesignChinese Academy of SciencesBeijing 100101China College of Life SciencesUniversity of Chinese Academy of SciencesBeijing 100101China College of Advanced Agricultural SciencesUniversity of Chinese Academy of SciencesBeijing 100049China 

出 版 物：《National Science Open》 (国家科学进展（英文）)

年 卷 期：2023年第2卷第5期

页      面：11-24页

学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：supported by the National Natural Science Foundation of China(31788103 and 31971370) the National Key Research and Development Program(2022YFF1002802) the Ministry of Agriculture and Rural Affairs of China,and the Strategic Priority Research Program of the Chinese Academy of Sciences(Precision Seed Design and Breeding,XDA24020102) 

主　　题：genome editing Agrobacterium infiltration anthocyanin transient transformation geminivirus replicon strawberry tomato 

摘      要：CRISPR-based genome editing technologies continue to drive major advances in life sciences.A major challenge for realizing widespread use of genome editing in plants and agriculture is establishing methods that enable the rapid,comprehensive,and precise evaluation of editing technologies using transient *** we report a new and rapid genome editing evaluation method using Agrobacterium infiltration techniques to enable broad-spectrum,simplistic,and precise assessments of genome editing *** employed an anthocyanin marker to facilitate visual screenings of genome-edited cells for use in adult strawberry fruits as well as tomato fruits,cotton leaves,and sugar beet *** this method,we demonstrate the ability to quickly measure genome editing efficiencies mediated by SpCas9,LbCas12a,A3A-PBE,ABE8e,and *** new method will allow researchers to rapidly and easily evaluate genome editing tools across a broad spectrum of plant species,further expediting the development of genome-edited agricultural crops.