Differential expression of antimicrobial peptides in human fungal keratitis
作者机构：Department of OphthalmologyUnion HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022Hubei ProvinceChina
出 版 物：《国际眼科杂志:英文版》 (International Journal of Ophthalmology(English edition))
年 卷 期：2023年第16卷第10期
页 面：1630-1635页
核心收录：
学科分类：1002[医学-临床医学] 100212[医学-眼科学] 10[医学]
基 金：Supported by the National Natural Science Foundation of China(No.82171025,No.82070934) the Fundamental Research Funds for the Central Universities(HUST:No.2019kfyXMBZ065) the Key Research and Development Program of Hubei Province(No.2021BCA146) the Clinical Research Foundation of Wuhan Union Hospital(No.2021xhlcyj03)
主 题：antimicrobial peptides fungal keratitis Aspergillus Fusarium
摘 要：AIM:To analyze a series of antimicrobial peptides(AMPs)in corneal tissue from individuals with fungal keratitis(FK)during the active phase of the fungus infection and after ***:Patients undergone lamellar keratoplasty for the treatment of severe FK or corneal scar had their corneal buttons *** real-time polymerase chain reaction(PCR)was used to ascertain the gene expression of human beta-defensin(HBD)-1,-2,-3,-9,S100A7,8,9,and ***:All AMPs’messenger ribonucleic acid(mRNA)expression was considerably elevated in all samples(n=12).In contrast to controls,where HBD-2,-3,and S100A7 mRNAs were expressed at very low levels,it was discovered that HBD-1,-9,S100A8,S100A9,and LL-37 were constitutively expressed in all healed samples(n=4).HBD-1,-2-3,S100A7,and LL-37 mRNAs were significantly increased in all active FK samples(n=8).The levels of HBD-9,S100A8,and S100A9 mRNAs were moderately upregulated in all active FK *** comparison showed that HBD-2 was significantly increased in Fusarium keratitis samples(n=5),and LL-37 mRNAs were significantly enhanced in Aspergillus keratitis samples(n=3).Whereas there was not significantly increased of HBD-1,-3,-9,S100A7,8,9 mRNA in Aspergillus keratitis samples compared with Fusarium keratitis ***:AMPs expression is increased in active FK,but not all AMPs are equally ***-2 and LL-37 expression levels are the highest,showing some specificity of AMP expression related to *** AMPs,particularly HBD-2 may play a significant role in Fusarium keratitis and LL-37 might be the key player in Aspergillus keratitis.
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