Combining nitrogen effects and metabolomics to reveal the response mechanisms to nitrogen stress and the potential for nitrogen reduction in maize

作     者：LU Yan-li SONG Gui-pei WANG Yu-hong WANG Luo-bin XU Meng-ze ZHOU Li-ping WANG Lei LU Yan-li;SONG Gui-pei;WANG Yu-hong;WANG Luo-bin;XU Meng-ze;ZHOU Li-ping;WANG Lei

作者机构：State Key Laboratory of Efficient Utilization of Arid and Semi-arid Arable Land in Northern China/Key Laboratory of Plant Nutrition and FertilizerMinistry of Agriculture and Rural Affairs/Institute of Agricultural Resources and Regional PlanningChinese Academy of Agricultural SciencesBeijing 100081P.R.China Tianjin Academy of Agricultural SciencesTianjin 300384P.R.China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2023年第22卷第9期

页      面：2660-2672页

核心收录：

学科分类：09[农学] 0901[农学-作物学] 

基　　金：support of the National Key R&D Program of China(2021YFD1700900) 

主　　题：long-term experiment nitrogen deficiency nitrogen excess metabolites UPLC-QTOF 

摘      要：The physiological and metabolic differences in maize under different nitrogen(N)levels are the basis of reasonable N management,which is vital in improving fertilizer utilization and reducing environmental *** this paper,on the premise of defining the N fertilizer efficiency and yield under different long-term N fertilization treatments,the corresponding differential metabolites and their metabolic pathways were analyzed by untargeted metabolomics in maize.N stress,including deficiency and excess,affects the balance of carbon(C)metabolism and N metabolism by regulating C metabolites(sugar alcohols and tricarboxylic acid(TCA)cycle intermediates)and N metabolites(various amino acids and their derivatives).L-alanine,L-phenylalanine,L-histidine,and L-glutamine decreased under N deficiency,and L-valine,proline,and L-histidine increased under N *** addition to sugar alcohols and the above amino acids in C and N metabolism,differential secondary metabolites,flavonoids(e.g.,kaempferol,luteolin,rutin,and diosmetin),and hormones(e.g.,indoleacetic acid,trans-zeatin,and jasmonic acid)were initially considered as indicators for N stress diagnosis under this experimental *** study also indicated that the leaf metabolic levels of N2(120 kg ha–1 N)and N3(180 kg ha–1 N)were similar,consistent with the differences in their physiological indexes and yields over 12 *** study verified the feasibility of reducing N fertilization from 180 kg ha–1(locally recommended)to 120 kg ha–1 at the metabolic level,which provided a mechanistic basis for reducing N fertilization without reducing yield,further improving the N utilization rate and protecting the ecological environment.