Expression analyses of stress‑responsive genes in the hermatypic coral Acropora tenuis and its symbiotic dinofagellates after exposure to the herbicide Diuron

作     者：Hiroshi Ishibashi Seigo Minamide Ichiro Takeuchi 

作者机构：Graduate School of AgricultureEhime UniversityMatsuyamaEhime 790-8566Japan Center of Advanced Technology for the EnvironmentGraduate School of AgricultureEhime UniversityMatsuyamaEhime 790-8566Japan 

出 版 物：《Marine Life Science & Technology》 (海洋生命科学与技术（英文）)

年 卷 期：2023年第5卷第3期

页      面：289-299页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 070703[理学-海洋生物学] 0707[理学-海洋科学] 

基　　金：This work was supported by the Japan Society for the Promotion of Science(JSPS)Grants-in-Aid for Scientifc Research(KAKENHI)(Grant Nos.26281048 and 21H03619). 

主　　题：Acropora tenuis Calreticulin Coral bleaching Diuron Heat shock protein 

摘      要：Diuron is one of the most frequently applied herbicides in sugarcane farming in southern Japan,and Australia.In addition,it is used as a booster substance in copper-based antifouling paints.Due to these various uses,Diuron is released into the marine environment;however,little information is available on gene expression in corals and their symbiotic algae exposed to Diuron.We investigated the efects of Diuron on stress-responsive gene expression in the hermatypic coral Acropora tenuis and its symbiotic dinofagellates.After seven days of exposure to 1µg/L and 10µg/L Diuron,no signifcant changes in the body colour of corals were observed.However,quantitative reverse transcription-polymerase chain reaction analyses revealed that the expression levels of stress-responsive genes,such as heat shock protein 90(HSP90),HSP70,and calreticulin(CALR),were signifcantly downregulated in corals exposed to 10µg/L of Diuron for seven days.Moreover,aquaglyceroporin was signifcantly downregulated in corals exposed to environmentally relevant concentrations of 1µg/L Diuron.In contrast,no such efects were observed on the expression levels of other stress-responsive genes,such as oxidative stress-responsive proteins,methionine adenosyltransferase,and green/red fuorescent proteins.Diuron exposure had no signifcant efect on the expression levels of HSP90,HSP70,or HSP40 in the symbiotic dinofagellates.These results suggest that stress-responsive genes,such as HSPs,respond diferently to Diuron in corals and their symbiotic dinofagellates and that A.tenuis HSPs and CALRs may be useful molecular biomarkers for predicting stress responses induced by the herbicide Diuron.