Whole-brain Optical Imaging:A Powerful Tool for Precise Brain Mapping at the Mesoscopic Level
作者机构：Huazhong University of Science and Technology-Suzhou Institute for BrainsmaticsJiangsu Industrial Technology Research InstituteSuzhou215123China Wuhan National Laboratory for OptoelectronicsHuazhong University of Science and TechnologyWuhan430074China
出 版 物：《Neuroscience Bulletin》 (神经科学通报（英文版）)
年 卷 期：2023年第39卷第12期
页 面：1840-1858页
核心收录：
学科分类：0710[理学-生物学] 07[理学] 071006[理学-神经生物学]
基 金：supported by the STI2030-Major Projects(2021ZD0201001 and 2021ZD0201000) the National Natural Science Foundation of China(81827901 and 32192412)
主 题：Whole-brain optical imaging Optical sectioning Micrometer resolution Brain connectome Neural circuits Neuron
摘 要：The mammalian brain is a highly complex network that consists of millions to billions of densely-interconnected *** dissection of neural circuits at the mesoscopic level can provide important structural information for understanding the *** approaches can achieve submicron lateral resolution and achieve“optical sectioningby a variety of means,which has the natural advantage of allowing the observation of neural circuits at the mesoscopic *** whole-brain optical imaging methods based on tissue clearing or histological sectioning surpass the limitation of optical imaging depth in biological tissues and can provide delicate structural information in a large volume of *** with various fluorescent labeling techniques,whole-brain optical imaging methods have shown great potential in the brain-wide quantitative profiling of cells,circuits,and blood *** this review,we summarize the principles and implementations of various whole-brain optical imaging methods and provide some concepts regarding their future development.
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