Transcriptional profiling of dental sensory and proprioceptive trigeminal neurons using single-cell RNA sequencing

作     者：Pa Reum Lee Jihoon Kim Heather Lynn Rossi Sena Chung Seung Yub Han Junhyong Kim Seog Bae Oh Pa Reum Lee;Jihoon Kim;Heather Lynn Rossi;Sena Chung;Seung Yub Han;Junhyong Kim;Seog Bae Oh

作者机构：Department of Neurobiology and PhysiologySchool of Dentistry and Dental Research InstituteSeoul National UniversitySeoulRepublic of Korea Genomics and Computational Biology Graduate GroupUniversity of PennsylvaniaPhiladelphiaPAUSA Department of PathobiologyUniversity of PennsylvaniaPhiladelphiaPAUSA Department of BiologyUniversity of PennsylvaniaPhiladelphiaPAUSA Brain Science InstituteKorea Institute of Science and Technology(KIST)SeoulRepublic of Korea 

出 版 物：《International Journal of Oral Science》 (国际口腔科学杂志（英文版）)

年 卷 期：2023年第15卷第3期

页      面：513-526页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 0711[理学-系统科学] 1003[医学-口腔医学] 07[理学] 08[工学] 

基　　金：supported by the National Research Foundation (NRF) of Korea funded by the Korean government (Ministry of Education, Science and Technology) 

主　　题：trigeminal specialized Together 

摘      要：Dental primary afferent(DPA)neurons and proprioceptive mesencephalic trigeminal nucleus(MTN)neurons,located in the trigeminal ganglion and the brainstem,respectively,are essential for controlling masticatory *** extensive transcriptomic studies on various somatosensory neurons,there is still a lack of knowledge about the molecular identities of these populations due to technical challenges in their circuit-validated ***,we employed high-depth single-cell RNA sequencing(scRNA-seq)in combination with retrograde tracing in mice to identify intrinsic transcriptional features of DPA and MTN *** transcriptome analysis revealed five major types of DPA neurons with cell type-specific gene enrichment,some of which exhibit unique mechano-nociceptive properties capable of transmitting nociception in response to innocuous mechanical stimuli in the ***,we discovered cellular heterogeneity within MTN neurons that potentially contribute to their responsiveness to mechanical stretch in the masseter muscle ***,DPA and MTN neurons represented sensory compartments with distinct molecular profiles characterized by various ion channels,receptors,neuropeptides,and ***,our study provides new biological insights regarding the highly specialized mechanosensory functions of DPA and MTN neurons in pain and proprioception.