TMED2 Induces Cisplatin Resistance in Breast Cancer via Targeting the KEAP1-Nrf2 Pathway

作     者：Chen LIANG Han-yong ZHANG Yi-qian WANG Ling-ang YANG Yu-sen DU Ying LUO Tong-cun ZHANG Yao XU Chen LIANG;Han-yong ZHANG;Yi-qian WANG;Ling-ang YANG;Yu-sen DU;Ying LUO;Tong-cun ZHANG;Yao XU

作者机构：College of Life Sciences and HealthWuhan University of Science and TechnologyWuhan 430070China School of StomatologyTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China 

出 版 物：《Current Medical Science》 (当代医学科学（英文）)

年 卷 期：2023年第43卷第5期

页      面：1023-1032页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported by the Scientific Research Plan of Department of Education of Hubei Province(No.B2021021) 

主　　题：TMED2 KEAP1 Nrf2 cisplatin resistance breast cancer 

摘      要：Objective Cisplatin is the first-line treatment for breast cancer,but it faces challenges of drug *** study investigated new molecular mechanisms underlying cisplatin resistance in breast *** We analyzed sequencing data from the TCGA database to identify potential associations between transmembrane emp24 protein transport domain containing 2(TMED2)and breast *** blotting,real-time PCR,CCK-8,and TUNEL assays were used to measure the effects and molecular mechanism of TMED2 on cisplatin resistance in MCF-7 and MDA-MB-231 cell *** TMED2 was overexpressed in breast cancer and associated with poor ***2 increased cisplatin resistance in breast cancer cells in vitro via promoting ubiquitination of Kelch-like ECH-associated protein 1(KEAP1),relieving inhibition of KEAP1 on nuclear factor erythroid 2-related factor 2(Nrf2),and increasing expression of downstream drug resistance related genes,such as heme oxygenase 1(HO-1)and NAD(P)H quinone oxidoreductase 1(NQO1).Conclusion We identified a new molecular mechanism by which TMED2 affects cisplatin resistance in breast *** results provide theoretical guidance for future clinical applications.