Bear bile powder alleviates Parkinson’s disease-like behavior in mice by inhibiting astrocyte-mediated neuroinflammation

作     者：WANG Lupeng BAI Yuyan TAO Yanlin SHEN Wei ZHOU Houyuan HE Yixin WU Hui HUANG Fei SHI Hailian WU Xiaojun WANG Lupeng;BAI Yuyan;TAO Yanlin;SHEN Wei;ZHOU Houyuan;HE Yixin;WU Hui;HUANG Fei;SHI Hailian;WU Xiaojun

作者机构：Shanghai Key Laboratory of Compound Chinese MedicinesThe Ministry of Education(MOE)Key Laboratory for Standardization of Chinese MedicinesThe State Administration of TCM(SATCM)Key Laboratory for New Resources and Quality Evaluation of Chinese MedicineInstitute of Chinese Materia MedicaShanghai University of Traditional Chinese MedicineShanghai 201203China 

出 版 物：《Chinese Journal of Natural Medicines》 (中国天然药物（英文版）)

年 卷 期：2023年第21卷第9期

页      面：710-720页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：the Organizational Key Research and Development Program of Shanghai University of Traditional Chinese Medicine(No.2023YZZ02) 

主　　题：Parkinson's disease Bear bile powder Astrocyte Neuroinflammation Takeda G protein-coupled receptor 5 

摘      要：Parkinson’s disease(PD)is a common neurodegenerative disease in middle-aged and elderly *** particular,increasing evidence has showed that astrocyte-mediated neuroinflammation is involved in the pathogenesis of *** a precious traditional Chinese medicine,bear bile powder(BBP)has a long history of use in clinical *** has numerous activities,such as clearing heat,calming the liver wind and anti-inflammation,and also exhibits good therapeutic effect on convulsive ***,whether BBP can prevent the development of PD has not been ***,this study was designed to explore the effect and mechanism of BBP on suppressing astrocyte-mediated neuroinflammation in a mouse model of ***-like behavior was induced in the mice by intraperitoneal injection of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)(30 mg·kg−1)for five days,followed by BBP(50,100,and 200 mg·kg−1)treatment daily for ten *** stimulated rat C6 astrocytic cells were used as a cell model of *** results indicated that BBP treatment significantly ameliorated dyskinesia,increased the levels of tyrosine hydroxylase(TH)and inhibited astrocyte hyperactivation in the substantia nigra(SN)of PD ***,BBP decreased the protein levels of glial fibrillary acidic protein(GFAP),cyclooxygenase 2(COX2)and inducible nitric oxide synthase(iNOS),and up-regulated the protein levels of takeda G protein-coupled receptor 5(TGR5)in the ***,BBP significantly activated TGR5 in a dose-dependent manner,and decreased the protein levels of GFAP,iNOS and COX2,as well as the mRNA levels of GFAP,iNOS,COX2,interleukin(IL)-1β,IL-6 and tumor necrosis factor-α(TNF-α)in LPS-stimulated C6 ***,BBP suppressed the phosphorylation of protein kinase B(AKT),inhibitor of NF-κB(IκBα)and nuclear factor-κB(NF-κB)proteins in vivo and in *** also observed that TGR5 inhibitor triamterene attenuated the anti-neuroinflammatory effect of BBP on LPS-stimulated C6 ***