Rapid visual detection of Vibrio parahaemolyticus by combining LAMP-CRISPR/Cas12b with heat-labile uracil-DNA glycosylase to eliminate carry-over contamination

作     者：Fang WU Chen LU Wenhao HU Xin GUO Jiayue CHEN Zhidan LUO Fang WU;Chen LU;Wenhao HU;Xin GUO;Jiayue CHEN;Zhidan LUO

作者机构：Jiangsu Key Laboratory of Marine Biological Resources and EnvironmentJiangsu Key Laboratory of Marine Pharmaceutical Compound ScreeningJiangsu Ocean UniversityLianyungang 222005China Co-Innovation Center of Jiangsu Marine Bio-industry TechnologyJiangsu Ocean UniversityLianyungang 222005China BestEnzymes Biotech Co.Ltd.Lianyungang 222005China 

出 版 物：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 (浙江大学学报（英文版）B辑（生物医学与生物技术）)

年 卷 期：2023年第24卷第8期

页      面：749-754页

核心收录：

学科分类：0710[理学-生物学] 1001[医学-基础医学(可授医学、理学学位)] 09[农学] 0906[农学-兽医学] 0836[工学-生物工程] 

基　　金：supported by the Priority Academic Program Development of Jiangsu Higher Education Institutions of China,the Postgraduate Research&Practice Innovation Program of Jiangsu Province(No.KYCX2021-038) the Lianyungang Science and Technology Program(No.CG2232). 

主　　题：LAMP visual system 

摘      要：Vibrio parahaemolyticus is a major pathogen frequently found in seafood.Rapid and accurate detection of this pathogen is important for the control of bacterial foodborne diseases and to ensure food safety.In this study,we established a one-pot system that combines uracil-DNA glycosylase(UDG),loopmediated isothermal amplification(LAMP),and clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 12b(Cas12b)for detecting V.parahaemolyticus in seafood.This detection system can effectively perform identification using a single tube and avoid the risk of carryover contamination.