Single-cell and spatial heterogeneity landscapes of mature epicardial cells
作者机构：Department of CardiologyThe Second Affiliated Hospital of Chongqing Medical UniversityChongqing400010China Department of NephrologyThe Second Affiliated Hospital of Chongqing Medical UniversityChongqing400010China Key Laboratory of Molecular Biology for Infectious DiseasesMinistry of EducationInstitute for Viral HepatitisThe Second Affiliated Hospital of Chongqing Medical UniversityChongqing400010China Precision Medicine CenterThe Second Affiliated Hospital of Chongqing Medical UniversityChongqing400010China Basic Medicine Research and Innovation Center for Novel Target and Therapeutic InterventionMinistry of EducationThe Second Affiliated Hospital of Chongqing Medical UniversityChongqing400010China
出 版 物：《Journal of Pharmaceutical Analysis》 (药物分析学报（英文版）)
年 卷 期：2023年第13卷第8期
页 面：894-907页
核心收录：
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金：supported by grants from the National Natural Science Foundation of China(Grant No.:82270281) Chongqing Medical University Program for Youth Innovation in Future Medicine(Grant No.:W0133) Senior Medical Talents Program of Chongqing for Young and Middle-aged,China(Program No.:JianlinDu) Postdoctoral Research Funding of the Second Affiliated Hospital of Chongqing Medical University,China(Grant No.:rsc-postdoctor114) and Kuanren Talents Program of the Second Affiliated Hospital of Chongqing Medical University,China(Program No.:kryc-gg-2102)
主 题：Epicardial cells Gene markers Single-cell sequencing Spatial transcriptomics
摘 要：Tbx18,Wt1,and Tcf21 have been identified as epicardial markers during the early embryonic ***,the gene markers of mature epicardial cells remain ***-cell transcriptomic analysis was performed with the Seurat,Monocle,and CellphoneDB packages in R software with standard *** transcriptomics was performed on chilled Visium Tissue Optimization Slides(10x Genomics)and Visium Spatial Gene Expression Slides(10x Genomics).Spatial transcriptomics analysis was performed with Space Ranger software and R ***,whole-mount RNA in situ hybridization and X-gal staining were performed to validate the analysis *** transcriptomics analysis revealed distinct transcriptional profiles and functions between epicardial tissue and non-epicardial *** gene markers specific to postnatal epicardial tissue were identified,including Msln,C3,Efemp1,and ***-cell transcriptomic analysis revealed that cardiac cells from wildtype mouse hearts(from embryonic day 9.5 to postnatal day 9)could be categorized into six major cell types,which included epicardial *** epicardial development,Wt1,Tbx18,and Upk3b were consistently expressed,whereas genes including Msln,C3,and Efemp1 exhibited increased expression during the mature stages of *** analysis further revealed two epicardial cell fates during ***,Upk3b,Msln,Efemp1,and C3 positive epicardial cells were enriched in extracellular matrix *** results suggested Upk3b,Efemp1,Msln,C3,and other genes were mature epicardium *** matrix signaling was found to play a critical role in the mature epicardium,thus suggesting potential therapeutic targets for heart regeneration in future clinical practice.
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