Rapid and sensitive detection of Epstein-Barr virus antibodies in nasopharyngeal carcinoma by chemiluminescence strips based on iron-porphyrin single atom nanozyme

作     者：Daji Wang Jie Wang Dan Liu Jiuyang He Meiying Wang Haibing Huang Guohui Nie Hui Ding Xiyun Yan 

作者机构：Nanozyme Synthesis CenterShenzhen Institute of Synthetic BiologyShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen 518055China Center for Drug EvaluationNational Medical Products AdministrationBeijing 100022China Shenzhen Key Laboratory of nanozymes and Translational Cancer ResearchDepartment of Otolaryngologyand Shenzhen Institute of Translational MedicineShenzhen Second People’s Hospital/the First Affiliated Hospital of Shenzhen University Health Science CenterShenzhen 518035China CAS Engineering Laboratory for NanozymeKey Laboratory of Protein and Peptide PharmaceuticalInstitute of BiophysicsChinese Academy of SciencesBeijing 100101China Department of Rheumatology and ImmunologyShenzhen Second People’s HospitalThe First Affiliated Hospital of Shenzhen UniversityShenzhen 518055China 

出 版 物：《Nano Research》 (纳米研究（英文版）)

年 卷 期：2024年第17卷第3期

页      面：1827-1836页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported by the National Key Research and Development Program of China(No.2019YFA0709204) the National Natural Science Foundation of China(No.32200744) the Natural Science Foundation of Guangdong Province(Nos.2021A1515110028 and 2022A1515220147) the Science and Technology Program for Basic Research in Shenzhen(Nos.JCYJ20210324103015039,JCYJ20190809095811254,and JCYJ20200109140412476) the National Natural Science Foundation of China(Nos.81930050 and 22121003) 

主　　题：iron-porphyrin single-atom nanozyme(SANzyme) chemiluminescence strips Epstein-Barr virus(EBV) nasopharyngeal carcinoma(NPC) antibody detection 

摘      要：The correlation between Epstein-Barr virus(EBV)infection and nasopharyngeal carcinoma(NPC)risk has been extensively *** continual monitoring of EBV-IgAs provides a promising approach of NPC screening in its early *** this study,we successfully synthesized a single-atom nanozyme(SANzyme)through the application of iron-porphyrin based metal organic framework(MOF-FeP).The MOF-FeP possesses precisely-defined electronic and geometric structures that accurately mimic highly-evolved catalytic site of natural *** peroxidase-like activity of MOF-FeP enables it to catalyze the chemiluminescence of luminol *** integrating MOF-FeP into a traditional strip,we created a rapid and highly-sensitive evaluation tool for detecting ***,the MOF-FeP strip enables the simultaneous detection of three EBV-IgAs,greatly improving the accuracy of EBV-associated NPC *** sensitivities of the MOF-FeP strip(75.56%–93.30%)surpass those of current enzyme-linked immunosorbent assay(ELISA)methods(64.44%–82.22%).This test takes only 16 min to perform as opposed to the customary 1–2 h required for standard ***,the MOF-FeP strip is suitable for whole blood samples,thereby significantly simplifying the sample preparation and detection *** conclusion,the MOF-FeP strip combines the simplicity of traditional strip with the high catalytic activity of *** innovative MOF-FeP strip offers a new point-of-care strategy for EBV-IgAs detection,which is expected to markedly facilitate early screening for EBV-associated diseases.