Microcurvature landscapes induce neural stem cell polarity and enhance neural differentiation
作者机构：Department of Biomedical EngineeringThe Hong Kong Polytechnic UniversityHung HomKowloonHong Kong SARChina State Key Laboratory of Ultra-Precision Machining TechnologyDepartment of Industrial and Systems EngineeringThe Hong Kong Polytechnic UniversityHung HomKowloonHong Kong SARChina
出 版 物：《生物设计与制造(英文)》 (Bio-Design and Manufacturing)
年 卷 期：2023年第6卷第5期
页 面：522-535页
核心收录：
学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 0710[理学-生物学] 1002[医学-临床医学] 08[工学] 0805[工学-材料科学与工程（可授工学、理学学位）]
基 金：supported by the Inter-Departmental Open Project of State Key Laboratory in Ultra-Precision Machining Technology(SKL-UPMT No.P0033576).
主 题：Curvature Neural differentiation Neurite outgrowth Mechanotransduction
摘 要：Tissue curvature has long been recognized as an important anatomical parameter that affects intracellular behaviors,and there is emerging interest in applying cell-scale curvature as a designer property to drive cell fates for tissue engineering purposes.Although neural cells are known to undergo dramatic and terminal morphological changes during development and curvature-limiting behaviors have been demonstrated in neurite outgrowth studies,there are still crucial gaps in understanding neural cell behaviors,particularly in the context of a three-dimensional(3D)curvature landscape similar to an actual tissue engineering scaffold.In this study,we fabricated two substrates of microcurvature(curvature-substrates)that present a smooth and repeating landscape with focuses of either a concave or a convex pattern.Using these curvature-substrates,we studied the properties of morphological differentiation in N2a neuroblastoma cells.In contrast to other studies where two-dimensional(2D)curvature was demonstrated to limit neurite outgrowth,we found that both the concave and convex substrates acted as continuous and uniform mechanical protrusions that significantly enhanced neural polarity and differentiation with few morphological changes in the main cell body.This enhanced differentiation was manifested in various properties,including increased neurite length,increased nuclear displacement,and upregulation of various neural markers.By demonstrating how the micron-scale curvature landscape induces neuronal polarity,we provide further insights into the design of biomaterials utilizing the influence of surface curvature in neural tissue engineering.
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