Identification of potential indicators for testis quality in swamp eels(Monopterus albus)through metabolomics analysis
作者机构：Key Laboratory of Breeding Biotechnology and Sustainable AquacultureInstitute of HydrobiologyThe Innovative Academy of Seed DesignHubei Hongshan LaboratoryChinese Academy of SciencesWuhan430072China College of Advanced Agricultural SciencesUniversity of Chinese Academy of SciencesBeijing100049China Guangdong Laboratory for Lingnan Modern AgricultureGuangzhou510642China
出 版 物：《Water Biology and Security》 (水生生物与安全(英文))
年 卷 期：2023年第2卷第4期
页 面：78-90页
学科分类：0710[理学-生物学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 0908[农学-水产] 07[理学] 0713[理学-生态学]
基 金：supported by grants from the National Natural Science Foundation of China(No.31922085) Natural Science Foundation of Hubei Province(Nos.2020CFA056 and 2022BBA0010) Foundation of Hubei Hongshan Laboratory(2022hszd022)to D Luo
主 题：Monopterus albus Sperm motility Testis quality Metabolomics Nucleotide metabolites
摘 要：The swamp eel(Monopterus albus)is a commonly cultured freshwater *** and cultivation of fecund broodstock is crucial for efficient artificial reproduction in this ***,there are currently no guidelines for selecting high-quality males in *** study quantitatively investigated the sperm motility of male swamp eels during the breeding season by using computer-assisted sperm analysis *** in the proportion and number of motile sperm was observed between individual *** testes were grouped into high-quality and low-quality according to sperm *** parameters of the two groups were *** found that high-quality testes were longer(150±27 mm verses 127±19 mm)than low-quality ***,the proportion of testes with a dark appearance was slightly higher(58.3%verses 48.1%)in the high-quality group than in the low-quality *** analysis was conducted to compare the metabolite profiles of high-quality and low-quality ***,organic acids,glycerolipids,amino acids,sphingolipids,and nucleotides were the most abundant metabolites in the testes of swamp ***,135 differential metabolites were *** nucleotide derivatives,including 50-deoxyadenosine,20-deoxyadenosine,and ribosyl adenosine,were the most significantly enriched metabolites in highquality *** metabolites are potential indicators for the testis quality in ***,our work provides systematic and quantitative data about the physiological and biochemical characteristics of *** testis,which provides an evidential basis for the selection of high-fecundity parents to improve artificial reproduction efficiency of ***.
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