Multi-omics analysis reveals the evolutionary origin of diterpenoid alkaloid biosynthesis pathways in Aconitum

作     者：Dake Zhao Ya Zhang Huanxing Ren Yana Shi Ding Dong Zonghang Li Guanghong Cui Yong Shen Zongmin Mou Edward JKennelly Luqi Huang Jue Ruan Suiyun Chen Diqiu Yu Yupeng Cun Dake Zhao;Ya Zhang;Huanxing Ren;Yana Shi;Ding Dong;Zonghang Li;Guanghong Cui;Yong Shen;Zongmin Mou;Edward J.Kennelly;Luqi Huang;Jue Ruan;Suiyun Chen;Diqiu Yu;Yupeng Cun

作者机构：School of Ecology and Environmental ScienceYunnan UniversityKunming 650500China Agricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518000China Chongqing Key Laboratory of Translational Medical Research in Cognitive Development and Learning and Memory DisordersPediatric Research InstituteMinistry of Education Key Laboratory of Child Development and DisordersNational Clinical Research Center for Child Health and DisordersChina International Science and Technology Cooperation Base of Child Development and Critical DisordersChildren's Hospital of Chongqing Medical UniversityChongqing 400014China Germplasm Bank of Wild SpeciesKunming Institute of BotanyChinese Academy of SciencesKunming 650201China Faculty of Life Science and TechnologyKunming University of Science and TechnologyKunming 650500China National Resource Center for Chinese Materia MedicaChinese Academy of Chinese Medical SciencesBeijing 100700China College of Agriculture and BiotechnologyYunnan Agricultural UniversityKunming 650201China Department of Biological SciencesLehman CollegeCity University of New YorkBronx 10468New YorkUSA Graduate CenterCity University of New YorkBronx 10468New YorkUSA State Key Laboratory for Conservation and Utilization of Bio‐resources in YunnanYunnan UniversityKunming 650500China 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报(英文版))

年 卷 期：2023年第65卷第10期

页      面：2320-2335页

核心收录：

学科分类：0710[理学-生物学] 1008[医学-中药学(可授医学、理学学位)] 10[医学] 

基　　金：supported by funding from the CAS “Pioneer Hundred Talents” Program and Strategic Priority Research Program of the Chinese Academy of Sciences (XDB31000000) to Y.C National Natural Science Foundation of China (Nos. 31960082, 32270395, and 31960096) Yunnan Fundamental Research Projects (No. 202101AS070021) to D.Z 

主　　题：Aconitum vilmorinianum acute toxicity biosynthetic pathway diterpenoid alkaloid evolutionary origin genome assembly 

摘      要：Diterpenoid alkaloids(DAs) have been often utilized in clinical practice due to their analgesic and anti-infammatory properties. Natural DAs are prevalent in the family Ranunculaceae, notably in the Aconitum genus. Nevertheless, the evolutionary origin of the biosynthesis pathway responsible for DA production remains *** this study, we successfully assembled a highquality, pseudochromosome-level genome of the DA-rich species Aconitum vilmorinianum(***)(5.76 Gb). An A. vilmorinianumspecific whole-genome duplication event was discovered using comparative genomic analysis,which may aid in the evolution of the DA biosynthesis pathway. We identified several genes involved in DA biosynthesis via integrated genomic, transcriptomic, and metabolomic analyses. These genes included enzymes encoding target ent-kaurene oxidases and aminotransferases, which facilitated the activation of diterpenes and insertion of nitrogen atoms into diterpene skeletons, thereby mediating the transformation of diterpenes into DAs. The divergence periods of these genes in A. vilmorinianum were further assessed, and it was shown that two major types of genes were involved in the establishment of the DA biosynthesis pathway. Our integrated analysis offers fresh insights into the evolutionary origin of DAs in *** as well as suggestions for engineering the biosynthetic pathways to obtain desired DAs.